Study of the effect of obstructive sleep apnea on the telomere length and its associated mechanisms

• Grant number: 18/00955-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): May 01, 2018
• Effective date (End): April 30, 2022
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Sergio Tufik
• Grantee: Priscila Farias Tempaku
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Obstructive Sleep Apnea (OSA) is a highly prevalent and morbid sleep disorder. Among the factors associated with its pathophysiology, the role of intermittent hypoxia stands out, contributing to the development of oxidative stress and inflammation. It is known that cumulative levels of these factors negatively influence the final portion of the DNA, known as telomere. In this sense, we hypothesize that OSA is capable of accelerating aging process through telomere shortening mediated by inflammatory and oxidative markers. Thus, the aim of this study is to investigate the effect of OSA and its treatment with CPAP on the variation of telomere length and their associated mechanisms. For this, a randomized, double-blind, sham-controlled clinical study with 6 months duration will be conducted. We will recruit 76 male participants with OSA diagnosis (apnea-hypopnea indexe15/hour), aged between 35-65 years and body mass index <35 kg/m2, which will be randomized to use CPAP or sham-CPAP for 6 months. Participants will visit the laboratory 6 times (baseline and after 1, 4, 6, 12 and 24 weeks) and they will be submitted to clinical and otorhinolaryngological evaluation, sleep questionnaires, polysomnography and blood collection for DNA and extraction and measurement of telomere length, as well as the expression of telomerase and oxidative and inflammatory markers (ADMA, homocysteine, cysteine, TBARS, 8-oxodG, TNF-a, IL-6 and IL-10). This project aims to contribute to the elucidation of the effect of OSA on telomere length maintenance, as well as the adjacent mechanisms to this relationship. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEMPAKU, PRISCILA FARIAS; D'ALMEIDA, VANIA; TUFIK, SERGIO. Re: ``Decreased Levels of Anti-Aging Klotho in Obstructive Sleep Apnea{''} by Pako et al. (Rejuvenation Res 2019 [Epub ahead of print]; DOI: 10.1089/rej.2019.2183). REJUVENATION RESEARCH, v. 22, n. 4, p. 364-365, AUG 1 2019. (18/00955-4)

TEMPAKU, PRISCILA FARIAS; E SILVA, LUCIANA OLIVEIRA; GUIMARAES, THAIS MOURA; VIDIGAL, TATIANA DE AGUIAR; D'ALMEIDA, VANIA; ANDERSEN, MONICA LEVY; BITTENCOURT, LIA; TUFIK, SERGIO. The reproducibility of clinical OSA subtypes: a population-based longitudinal study. Sleep and Breathing, JAN 2022. (18/00955-4)

TEMPAKU, PRISCILA FARIAS; SANTORO, MARCOS LEITE; BITTENCOURT, LIA; D'ALMEIDA, VANIA; BELANGERO, SINTIA IOLE; TUFIK, SERGIO. Genome-wide association study reveals two novel risk alleles for incident obstructive sleep apnea in the EPISONO cohort. Sleep Medicine, v. 66, p. 24-32, FEB 2020. (18/00955-4)

TEMPAKU, PRISCILA FARIAS; D'ALMEIDA, VANIA; AFFONSO DA SILVA, SYLVIA MARIA; ANDERSEN, MONICA LEVY; BELANGERO, SINTIA IOLE; TUFIK, SERGIO. Klotho genetic variants mediate the association between obstructive sleep apnea and short telomere length. Sleep Medicine, v. 83, p. 210-213, JUL 2021. (18/00955-4)

TEMPAKU, PRISCILA FARIAS; D'ALMEIDA, VANIA; TUFIK, SERGIO. Re: "Decreased Levels of Anti-Aging Klotho in Obstructive Sleep Apnea" by Pako et al. (Rejuvenation Res 2019 [Epub ahead of print]; DOI: 10.1089/rej.2019.2183). REJUVENATION RESEARCH, v. 22, n. 4, p. 2-pg., 2019-08-01. (18/00955-4)