Anticancer potential of isolated substances from Streptomyces SP. recovered from ascidian Euherdmania SP

• Grant number: 17/18235-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2018
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Leticia Veras Costa Lotufo
• Grantee: Luciana Costa Furtado
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds, AP.BTA.TEM

Abstract

Natural products of marine origin (PNM) emerge as a source of invaluable pharmacological potential. In this perspective, the sustainable supply of bioactive metabolites from the cultivation of marine microorganisms appears as an attractive solution for the rational prospection of PNM. Endemic Brazilian ascidia, Euherdmania sp., has been shown to be a promising source of microorganisms that produce cytotoxic substances. Previous studies have shown that the strain BRA-346, belonging to the genus Streptomyces, showed the most active extract with IC50 of 30ng/mL in human HCT-116 colorectal carcinoma cells. This extract also showed potent inhibition of the catalytic activity of the proteasome, by means of a target-directed assay using crystallography (IC50= 0.42ug/mL). Proteasome inhibitors are used clinically in the treatment of multiple myeloma, but have been tested with promising results in glioblastoma cells. Therefore, the objective of the present project will be to evaluate the mechanisms involved in cytotoxicity in glioblastoma cells and inhibition of proliferation of cells treated with the proteasome inhibitor (s) present in the BRA-346 extract. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PENA-HIDALGO, MIGUEL; FURTADO, LUCIANA COSTA; COSTA-LOTUFO, LETICIA V.; FERREIRA, MARCELO J. P.; SANTOS, DEBORAH Y. A. C.. Alkaloids from the Leaves of Annona crassiflora and Their Cytotoxic Activity. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 31, n. 2, p. 244-248, APR 2021. (17/18235-5, 15/17177-6, 07/06511-6, 13/07543-0)

TANGERINA, MARCELO M. P.; FURTADO, LUCIANA COSTA; LEITE, VIDA M. B.; BAUERMEISTER, ANELIZE; VELASCO-ALZATE, KAREN; JIMENEZ, PAULA C.; GARRIDO, LEANDRO M.; PADILLA, GABRIEL; LOPES, NORBERTO P.; COSTA-LOTUFO, LETICIA V.; et al. Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds. PLoS One, v. 15, n. 12, DEC 21 2020. (17/16606-6, 15/17177-6, 17/18235-5, 17/17648-4)

NISHIMURA, V, RODOLFO H.; DOS SANTOS, THIAGO; MURIE, VALTER E.; FURTADO, LUCIANA C.; COSTA-LOTUFO, V, LETICIA; CLOSOSKI, GIULIANO C.. Efficient N-arylation of 4-chloroquinazolines en route to novel 4-anilinoquinazolines as potential anticancer agents. Beilstein Journal of Organic Chemistry, v. 17, p. 2968-2975, DEC 22 2021. (15/17177-6, 17/18235-5, 15/21364-6, 18/14150-8, 20/08987-2, 15/01466-9)

FURTADO, LUCIANA C.; BAUERMEISTER, ANELIZE; DE FELICIO, RAFAEL; ORTEGA, RAQUEL; PINTO, FRANCISCO DAS CHAGAS L.; MACHADO-NETO, JOAO AGOSTINHO; TRIVELLA, DANIELA B. B.; PESSOA, OTILIA D. L.; WILKE, DIEGO V.; LOPES, NORBERTO P.; et al. Marine Streptomyces sp. Isolated From the Brazilian Endemic Tunicate Euherdmania sp. Produces Dihydroeponemycin and Analogs With Potent Antiglioma Activity. FRONTIERS IN MARINE SCIENCE, v. 8, MAY 3 2021. (18/24865-4, 17/18235-5, 15/17177-6, 17/17648-4)

VIEIRA, BRUNA DOMINGUES; NIERO, HENRIQUE; DE FELICIO, RAFAEL; ALVES, LUIZ FERNANDO GIOLO; BAZZANO, CRISTINA FREITAS; SIGRIST, RENATA; FURTADO, LUCIANA COSTA; PERSINOTI, GABRIELA FELIX; COSTA-LOTUFO, LETICIA VERAS; TRIVELLA, DANIELA BARRETTO BARBOSA. Production of Epoxyketone Peptide-Based Proteasome Inhibitors by Streptomyces sp. BRA-346: Regulation and Biosynthesis. FRONTIERS IN MICROBIOLOGY, v. 13, p. 17-pg., 2022-03-24. (20/08987-2, 17/18235-5, 19/27306-9, 15/17177-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FURTADO, Luciana Costa. Potencial anticâncer de substâncias isoladas de Streptomyces sp. recuperada da ascídia Euherdmania sp.. 2019. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.