Using next-generation sequencing data to estimate ploidy level and access cyto-nuclear genomic variability of a neotropical species complex of unisexual lizards (Squamata: Gymnophthalmidae: Loxopholis)

• Grant number: 18/07660-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: August 15, 2018
• End date: February 14, 2019
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Miguel Trefaut Urbano Rodrigues
• Grantee: Tuliana Oliveira Brunes
• Supervisor: Matthew Kenji Fujita
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Texas at Arlington (UT Arlington), United States
• Associated to the scholarship: 16/03146-4 - Unveiling geographical, ecological, and evolutionary relationships of a Neotropical species complex of unisexual lizards of genus Leposoma (Squamata: Gymnophthalmidae) through Ecological Niche Modeling, BP.PD

Abstract

Neotropical lizards from Loxopholis percarinatum (s.n. Leposoma percarinatum) complex are intriguing because they present a unisexual reproductive mode, including individuals with dif-ferent ploidy numbers (2n and 3n). The origin and impacts of sex-independent reproduction and the increase of ploidy has long been fascinating evolutionary biologists. With the post-doctoral project (#2016/03146-4), we have been investigating the mechanisms behind the origin of par-thenogenesis in L. percarinatum, and addressing their ecological and biogeographic impacts. For that, we have been performing a set of Ecological Niche Modeling analyses and combining these data with the phylogenetic history. Our first results clarified the type and species involved in the origin of parthenogenesis in Loxopholis. Additionally, we also find signs of recent hybrid-ization between parthenogenetic females and males from one of the progenitor species in a sympatric region located in the west of the Rio Negro. Although both results are unpublished, they also brought some challenges, described in the second project report, related with the ploidy and genetic variability of individuals. To solve that, in this project we propose to collect and process a large number of nuclear markers through a genome-wide association study, using Restriction site-associated DNA sequencing in the Dr. Matthew Fujita laboratory, University of Texas at Arlington, United States. To take advantage of the internship, additionally, we will also gather mitochondrial genomic data to study the organelle molecular evolution of. L. percarinatum complex. Finally, these data will allow us to robstly validate the ancestry of the L. percarinatum complex and perform more precisely the ecological niche comparison proposed in the initial project.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRUNES, TULIANA O.; LYRA, MARIANA L.; MALDONADO, JOSE A.; PELLEGRINO, KATIA C. M.; RODRIGUES, MIGUEL TREFAUT; FUJITA, MATTHEW K.. The first mitochondrial genome of a South America parthenogenetic lizard (Squamata: Gymnophthalmidae). MITOCHONDRIAL DNA PART B-RESOURCES, v. 6, n. 8, p. 2393-2395, AUG 3 2021. (16/03146-4, 13/50297-0, 11/50146-6, 18/07660-0)

FUJITA, MATTHEW K.; SINGHAL, SONAL; BRUNES, TULIANA O.; MALDONADO, JOSE A.; FUTUYMA, DJ. Evolutionary Dynamics and Consequences of Parthenogenesis in Vertebrates. ANNUAL REVIEW OF ECOLOGY, EVOLUTION, AND SYSTEMATICS, VOL 51, 2020, v. 51, p. 24-pg., 2020-01-01. (16/03146-4, 18/07660-0)