Recovering, modernizing and expanding the collection of herpetology at Butantan Institute through integrative studies on systematics and evolution of neotropical snakes

• Grant number: 22/12660-4
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
• Start date: April 01, 2023
• End date: March 31, 2028
• Field of knowledge: Biological Sciences - Zoology - Taxonomy of Recent Groups
• Principal Investigator: Felipe Gobbi Grazziotin
• Grantee: Felipe Gobbi Grazziotin
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Adrian Antonio Garda ; Ana Lúcia da Costa Prudente ; Ana Maria Moura da Silva ; Andrew James Mason ; Carlos Alberto Gonçalves Silva Jared ; Christian David Salazar Valenzuela ; Christopher Laird Parkinson ; Darin Rokyta ; Harold Lisle Gibbs ; Inácio de Loiola Meirelles Junqueira de Azevedo ; Márcio Borges Martins ; Marta Maria Antoniazzi ; Miguel Trefaut Urbano Rodrigues ; Pablo Javier Venegas Ibañez ; Paola Andrea Carrasco ; Paulo Gustavo Homem Passos ; Pedro Gabriel Nachtigall ; Ricardo Jannini Sawaya ; Taran Grant ; Wilmar Bolivar Garcia
• Associated scholarship(s): 25/05668-7 - Taxonomic revision of Bothrops mattogrossensis Amaral, 1925 (Serpentes, Crotalinae), based on morphological and molecular data, BP.MS; 25/03049-8 - Fieldwork assistance for specimen, venom, and gland collection, curation of the specimen collection, tissues, venoms, and glands (first phase)., BP.TT; 24/15082-7 - Taxonomy and phylogenetic systematics of the species complex Tantilla melanocephala (Serpentes: Colubridae): investigating biogeographic patterns, unraveling evolutionary processes, BP.DR; + associated scholarships 24/09356-7 - Venomic and biological function assessment of Lachesis muta muta and Lachesis muta rhombeata venoms, BP.MS; 24/02785-0 - Bioinformatic support for transcriptomic data integration to the final stage of the Project Scales of Biodiversity, BP.TT; 23/12640-6 - Fieldwork assistance for specimen, venom, and gland collection, curation of the specimen collection, tissues, venoms, and glands (first phase)., BP.TT; 23/07927-4 - Fieldwork assistance for specimen, venom, and gland collection, curation of the specimen collection, tissues, venoms, and glands (first phase)., BP.TT - associated scholarships

Abstract

The scientific collections represent the institutions responsible for documenting, preserve and improve the knowledge about the world's biodiversity. Such knowledge is decisive for the development of science and societies, especially in times of environmental or health crises. The state of São Paulo, through the Butantan Institute, has one of the main collections of snakes in the world. In 2010, a major fire destroyed more than 80% of the Institute's Herpetological Collection. This proposal contemplates two distinct aspects: 1) recover, increase, and promote the use of biological collections; and 2) characterize patterns and processes that shape the biodiversity. Considering the first aspect, this proposal has three main objectives: 1) to restore, modernize and expand the Herpetological Collection of Instituto Butantan; 2) develop my research group at the Butantan Institute; and 3) make available and facilitate the access to information contained in the Herpetological Collection. For this, we will recover and modernize the access to the material of the collection through the sequencing of the type specimens (museomics) for all species of the genera Bothrops and Bothrocophias. We will also perform high-quality, high-contrast 3D imaging (micro-CT) of the same material. In parallel, we will expand the zoological collections through the field work expeditions targeting all type localities throughout South America, using a network of collaborators. Considering the second aspect, this proposal seeks to achieve two distinct and complementary goals: 1) perform integrative studies on systematics, biogeography and complex phenotypes (morphology and venom) of neotropical snakes, with emphasis on the genera Bothrops and Bothrocophias; and 2) to evaluate the processes of speciation and phenotypic evolution of species of Squamata present in the islands and on the coast of the state of São Paulo. For this, we will evaluate genomes, transcriptomics of venom gland and the morphological variation among insular species of the Bothrops, aiming the estimation of the effects of demography and natural selection in the generation of the genetic variability. We will also compare the evolutionary patterns and processes of these snakes with those present in other island squamata in the state of São Paulo. We will also evaluate the co-evolution between predators and prey through the study of venom and venom resistance in centipedes and insular snakes. Finally, we will describe the variability of Bothrops jararaca venom throughout its distribution through the evaluation of the transcriptome of venom glands and we will compare, through enzymatic assays, the biological activity of the venoms of all species of the Bothrops jararaca complex. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARRASCO, PAOLA A.; PRYSTUPCZUK, LILEN; KOCH, CLAUDIA; GONZALEZ, GERMAN A.; LEYNAUD, GERARDO C.; GRAZZIOTIN, FELIPE G.. Patterns of morphological variation and ecological correlates in the skull of vipers (Serpentes: Viperidae). Journal of Morphology, v. 284, n. 8, p. 17-pg., 2023-08-01. (22/12660-4, 16/50127-5)

ZAHER, HUSSAM; TRUSZ, CARLOS; KOCH, CLAUDIA; ENTIAUSPE-NETO, OMAR M.; BATTILANA, JAQUELINE; GRAZZIOTIN, FELIPE G.. Molecular phylogeny and biogeography of the dwarf boas of the family Tropidophiidae (Serpentes: Alethinophidia). SYSTEMATICS AND BIODIVERSITY, v. 22, n. 1, p. 28-pg., 2024-12-31. (02/13602-4, 16/50127-5, 11/50146-6, 21/13671-7, 22/12660-4)

ENTIAUSPE-NETO, OMAR M.; NACHTIGALL, PEDRO G.; BORGES-MARTINS, MARCIO; JUNQUEIRA-DE-AZEVEDO, INACIO L. M.; GRAZZIOTIN, FELIPE G.. Highly conserved and extremely variable: The paradoxical pattern of toxin expression revealed by comparative venom-gland transcriptomics of Phalotris (Serpentes: Dipsadidae). Toxicon, v. 244, p. 15-pg., 2024-05-15. (18/26520-4, 13/07467-1, 16/50127-5, 21/13671-7, 22/12660-4)

AZEVEDO, WEVERTON DOS SANTOS; FRANCO, FRANCISCO LUIS; MENEZES, LUCAS; KUNZ, TOBIAS SARAIVA; GRAZZIOTIN, FELIPE GOBBI. Integrated evidence sheds light on the taxonomy of the widespread Tantilla melanocephala species complex (Serpentes: Colubridae) and indicates the existence of a new species from southern South America. ORGANISMS DIVERSITY & EVOLUTION, v. 24, n. 1, p. 29-pg., 2024-01-24. (22/12660-4, 22/07935-4, 16/50127-5)

ENTIAUSPE-NETO, OMAR M.; DERVANOSKI, DARLAN; ABEGG, ARTHUR DIESEL. Can fieldwork driven by predictive species distribution models yield new rare or relevant geographic records? A case study with Neotropical snakes. AUSTRAL ECOLOGY, v. 49, n. 12, p. 16-pg., 2024-12-01. (16/50127-5, 21/13671-7, 22/12660-4)

ENTIAUSPE-NETO, OMAR M.; GIRAUDO, ALEJANDRO R.; GUEDES, THAIS B.; TIUTENKO, ARTHUR; BORGES-MARTINS, MARCIO; KOCH, CLAUDIA. Rediscovery of a rare and endangered Apostolepis (Serpentes: Dipsadidae): reassessing species boundaries and phylogenetic relationships using integrative approaches. SALAMANDRA, v. 61, n. 1, p. 28-pg., 2025-02-15. (22/09428-2, 16/50127-5, 22/12660-4, 21/13671-7, 21/07161-6)

FREITAS-DE-SOUSA, LUCIANA A.; COLOMBINI, MONICA; SOUZA, VINICIUS C.; SILVA, JOANDERSON P. C.; MOTA-DA-SILVA, AGEANE; ALMEIDA, MARLLUS R. N.; MACHADO, REGINALDO A.; FONSECA, WIRVEN L.; SARTIM, MARCO A.; SACHETT, JACQUELINE; et al. Venom Composition of Neglected Bothropoid Snakes from the Amazon Rainforest: Ecological and Toxinological Implications. TOXINS, v. 16, n. 2, p. 22-pg., 2024-02-01. (22/10344-8, 13/07467-1, 22/12660-4, 16/50127-5)