Proteomic and functional characterization of the venom of two species of snakes of the genus Bothrops submitted to diet change

• Grant number: 18/14724-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2018
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Karen de Morais Zani
• Grantee: Nathália da Costa Galizio
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated scholarship(s): 19/24527-4 - Compositional and functional characterization of the venom of two snake species from Bothrops genus that underwent a diet shift, BE.EP.MS

Abstract

Snake venoms are the most studied animal toxins. Its composition is complex, being composed mainly by proteins, which correspond to approximately 90% of its dry weight. Snake venom proteins are subject to Darwinian evolution and the variation in venom composition is observed at different taxonomic levels. Studies consistently demonstrate that there are large variations in the composition of venoms among different snake species and even within the same species. These differences may be due to varied factors, including gender, age, geographical distribution and diet. The variation in venom composition due to diet may be related to the specialization of the snake on different prey species. However, the relationship between diet and venom composition and activity is still controversial. In a previous study developed by our group, adult Bothrops moojeni individuals kept in the Herpetology Laboratory of the Butantan Institute had their diet changed from mammals (mice or rats), the standard diet used for the maintenance of the snakes in captivity, to amphibians (Lithobates catesbeianus). The results of this work, not published yet, showed a high intraspecific variation in the composition of the venom among the individuals and also a functional variation in the biological activities after the change of diet. Since Bothrops moojeni is a generalist species, which feeds on different prey categories, it is important to compare the plasticity of its venom to a specialist species, which feeds on only one type of prey throughout its life, as the species Bothrops alternatus. Therefore, the objective of this work is to investigate the influence of diet on the composition and biochemical and biological activities of Bothrops moojeni and Bothrops alternatus venoms.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO BRAGA, JACQUELINE RAMOS; DE MORAIS-ZANI, KAREN; PEREIRA, DIEGO DOS SANTOS; SANT'ANNA, SAVIO STEFANINI; GALIZIO, NATHALIA DA COSTA; TANAKA-AZEVEDO, ANITA MITICO; GOMES VILARINHO, ARIEL RODRIGUES; RODRIGUES, JOSE LUCCA; TEIXEIRA DA ROCHA, MARISA MARIA. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon, v. 184, p. 127-135, SEP 2020. (14/11108-0, 18/14724-4, 17/16908-2, 16/09976-9, 18/25786-0)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SANT'ANNA, SAVIO STEFANINI; SILVEIRA, GIOVANNI PEREZ M.; RODRIGUES, FABIOLA DE SOUZA; GREGO, KATHLEEN FERNANDES; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Ontogenetic study of Bothrops jararacussu venom composition reveals distinct profiles. Toxicon, v. 186, p. 67-77, OCT 30 2020. (17/16908-2, 18/25786-0, 18/14724-4, 18/20651-0)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations. PLoS One, v. 14, n. 9, SEP 12 2019. (17/01890-0, 17/16908-2, 14/11108-0, 17/20106-9, 18/20651-0, 18/14724-4, 18/25786-0)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; FABRI BITTENCOURT RODRIGUES, CAROLINE; STUGINSKI, DANIEL RODRIGUES; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; et al. From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages. PLoS One, v. 16, n. 6, JUN 10 2021. (17/16908-2, 17/20106-9, 18/25786-0, 18/25899-0, 18/14724-4)

LEANDRO NORBERTO DA SILVA-JÚNIOR; LARA DE SOUZA ABREU; CAROLINE FABRI BITTENCOURT RODRIGUES; NATHÁLIA DA COSTA GALIZIO; WESLEI DA SILVA AGUIAR; CAROLINE SERINO-SILVA; VALDOMIRO SOUZA DOS SANTOS; ISABELLA ALVES COSTA; LUIS VICENTE FRANCO OLIVEIRA; SÁVIO STEFANINI SANTANNA; et al. Geographic variation of individual venom profile of Crotalus durissus snakes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-08-26. (18/25786-0, 14/11108-0, 16/09976-9, 18/14724-4, 17/16908-2, 17/01890-0)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GALIZIO, Nathália da Costa. Proteomic and functional characterization of the venom of two species of snakes from the genus Bothrops submitted to diet change. 2021. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.