Decellularization of kidney pigs for production of scaffolds

• Grant number: 18/06030-2
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2018
• Effective date (End): December 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Maria Angelica Miglino
• Grantee: Igor Smirnow Cordeiro
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The chronic renal disease is characterized as an irreversible lesion that causes progressive loss of renal function and resulting in different stages of renal insufficiency. The more adequate treatment is the renal transplant, however, the organ availability for transplant is not the same as demand. Using organs to decellularization, an area of tissue engineering, could be an interest alternative for this finality. Therefore, this project aims in decellularize porcine kidneys, due similarity to human kidney size and structure, and validate their potential as biomaterial. In this study will be used 10 porcine kidneys to decellularization with 1% SDS and 1% Triton X-100 by perfusion method with ORCA Solid Organ Bioreactor (Harvard Apparatus). After decellularization process will be used several techniques to evaluate the structure (histology, scanning and transmission electron microscopy) and composition (immunohistochemistry and genomic DNA quantification) to validate as scaffold and their potential use as biomaterial.