Identification and quantification of antimicrobial resistance genes in the microbiome of seabirds from the Southern-Southeastern coast of Brazil

• Grant number: 16/20956-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2018
• End date: June 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Jose Luiz Catao Dias
• Grantee: Ana Carolina Ewbank
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/25885-9 - Identification and quantification of antimicrobial resistance genes in the microbiome of wild seabirds from Brazil: how do anthropogenic impacts affect these parameters?, BE.EP.DR

Abstract

The emergence of antibiotic resistance is considered a threat to human and animal health. Through horizontal trasfer, environmental antibiotic contamination may promote the exchange of genes between non-pathogenic and pathogenic environmental bacteria. The resulting remodeling of existing microbiomas leads to evolutionary and environmental complications that in association with their dissemination abilities classify genes of antibiotic resistance (ARGs) as environmental contaminants. Seabirds rapidly respond to environmental changes, being excellent marine ecossystem health biomarkers. Wildlife rehabilitation centers have a controverse role on the possible dissemination of bacteria presenting pathogenic genes and ARGs, both in the internal environment and external environment, after these animals are released. We intend on evaluating the degree of human interference in the marine ecossystem through the presence, identification and subsequent quantification of seleced ARGs (tetA, tetB, tetY, tetK, tetM, tetQ, sulI, sulII, str, aadA, catI, catII, erm(B), erm(F), qnrB, qnrS, blaCTX, blaTEM, mecA and mcr-1) present in plasmids of the microbiome of free ranging wild seabirds of two distinct groups: admitted in rehabilitation centers of the southern brazilian coast and residents of seabird colonies in the southeastern coast. For this purpose, we will employ real-time PCR (rtPCR) on fresh fecal samples (enema) collected (I) upon the individual's admission to the rehabilitation center; (II) 24 hours after initiation of the antimicrobial treatment; (III) 24 hrs after antimicrobial treatment termination and (IV) upon release, and of E. coli colonies previously isolated from several seabird species and stored in the Laboratory of Wildlife Comparative Pathology - LAPCOM/FMVZ/USP. Our goal is to determine the degree of environmental contamination by the ARGs present in seabirds at their habitat/admission, as well as the influence of antimicrobial treatment and/or stay in rehabilitation centers in regards to the presence and quantity of ARGs. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EWBANK, ANA CAROLINA; ESPERON, FERNANDO; SACRISTAN, CARLOS; SACRISTAN, IRENE; NEVES, ELENA; COSTA-SILVA, SAMIRA; ANTONELLI, MARZIA; LORENCO, JANAINA ROCHA; KOLESNIKOVAS, CRISTIANE K. M.; CATAO-DIAS, JOSE LUIZ. Occurrence and Quantification of Antimicrobial Resistance Genes in the Gastrointestinal Microbiome of Two Wild Seabird Species With Contrasting Behaviors. FRONTIERS IN VETERINARY SCIENCE, v. 8, MAR 22 2021. (16/20956-0, 18/25069-7)

DUARTE-BENVENUTO, ARICIA; SACRISTAN, CARLOS; EWBANK, ANA CAROLINA; SACRISTAN, IRENE; ZAMANA-RAMBLAS, ROBERTA; GRAVENA, WALESKA; MELLO, DANIELA M. D.; FERREIRA DA SILVA, VERA M.; MARMONTEL, MIRIAM; CARVALHO, VITOR L.; et al. Hemotropic Mycoplasma spp. in Aquatic Mammals, Amazon Basin, Brazil. Emerging Infectious Diseases, v. 28, n. 12, p. 4-pg., 2022-12-01. (18/25069-7, 16/20956-0)

EWBANK, ANA CAROLINA; ESPERON, FERNANDO; SACRISTAN, CARLOS; SACRISTAN, IRENE; KRUL, RICARDO; DE MACEDO, EDUARDO CAVALCANTE; CALATAYUD, OLGA; BUENO, IRENE; STREFEZZI, RICARDO DE FRANCISCO; CATAO-DIAS, JOSE LUIZ. Seabirds as anthropization indicators in two different tropical biotopes: A One Health approach to the issue of antimicrobial resistance genes pollution in oceanic islands. Science of The Total Environment, v. 754, FEB 1 2021. (18/25885-9, 18/25069-7, 16/20956-0)

COSTA-SILVA, SAMIRA; SACRISTAN, CARLOS; SOARES, RODRIGO M.; CARVALHO, VITOR L.; V. CASTILHO, PEDRO; CREMER, MARTA J.; EWBANK, ANA CAROLINA; DUARTE-BENVENUTO, ARICIA; FAITA, THALITA; NAVAS-SUAREZ, PEDRO E.; et al. Short-Finned Pilot Whale Strandings Associated with Pilot Whale Morbillivirus, Brazil. Emerging Infectious Diseases, v. 29, n. 1, p. 4-pg., 2023-01-01. (18/25069-7, 16/20956-0, 20/12434-9)

NOVOSELECKI, HELENA EXPOSTO; CATAO-DIAS, JOSE LUIZ; EWBANK, ANA CAROLINA; NAVAS-SUAREZ, PEDRO ENRIQUE; DUARTE-BENVENUTO, ARICIA; LIAL, HENRIQUE CHRISTINO; SILVA, SAMIRA COSTA; SANCHEZ-SARMIENTO, ANGELICA MARIA; GRAVENA, WALESKA; FERREIRA DA SILVA, VERA MARIA; et al. ighly divergent herpesviruses in threatened river dolphins from Brazi. SCIENTIFIC REPORTS, v. 11, n. 1, DEC 31 2021. (16/20956-0, 18/25069-7)

SACRISTAN, CARLOS; COSTA-SILVA, SAMIRA; REISFELD, LAURA; NAVAS-SUAREZ, PEDRO ENRIQUE; EWBANK, ANA CAROLINA; DUARTE-BENVENUTO, ARICIA; COELHO COUTO DE AZEVEDO FERNANDES, NATALIA; ALBERGARIA RESSIO, RODRIGO; ANTONELLI, MARZIA; ROCHA LORENCO, JANAINA; et al. Novel alphaherpesvirus in a wild South American sea lion (Otaria byronia) with pulmonary tuberculosis. Brazilian Journal of Microbiology, v. 52, n. 4, SEP 2021. (18/25069-7, 16/20956-0)

EWBANK, ANA CAROLINA; SACRISTAN, CARLOS; COSTA-SILVA, SAMIRA; ANTONELLI, MARZIA; LORENCO, JANAINA R.; NOGUEIRA, GUILHERME A.; EBERT, MARIANA B.; KOLESNIKOVAS, CRISTIANE K. M.; CATAO-DIAS, JOSE LUIZ. Postmortem findings in Magellanic penguins (Spheniscus magellanicus) caught in a drift gillnet. BMC Veterinary Research, v. 16, n. 1, MAY 24 2020. (18/25069-7, 16/20956-0)

REISFELD, LAURA; FERNANDES, NATALIA C. C. A.; SARMIENTO, ANGELICA; CANEDO, PALOMA; SALVAGNI, FERNANDA; EWBANK, ANA CAROLINA; BARRESE, TOMAS ZECCHINI; PONCE, CESAR CILENTO; RESSIO, RODRIGO ALBERGARIA; CATAO-DIAS, JOSE LUIZ; et al. Myeloid and histiocytic sarcomas in subantarctic fur seals Arctocephalus tropicalis, Brazil. DISEASES OF AQUATIC ORGANISMS, v. 148, p. 6-pg., 2022-01-01. (18/25069-7, 16/20956-0)

SACRISTAN, CARLOS; EWBANK, ANA CAROLINA; MARIA SANCHEZ-SARMIENTO, ANGELICA; DUARTE-BENVENUTO, ARICIA; GOMES BORGES, JOAO CARLOS; REBELO, VANESSA ARAUJO; DIAZ-DELGADO, JOSUE; KEID, LARA BORGES; CATAO-DIAS, JOSE LUIZ. Erysipelas in a stranded common bottlenose dolphin: first report in a South American odontocete. Brazilian Journal of Microbiology, v. 53, n. 4, p. 5-pg., 2022-08-12. (18/25069-7, 16/20956-0)

LIAL, HENRIQUE CHRISTINO; NAVAS-SUAREZ, PEDRO ENRIQUE; EWBANK, ANA CAROLINA; NOVOSELECKI, HELENA EXPOSTO; FERREIRA-MACHADO, EDUARDO; DOS SANTOS CIRQUEIRA, CINTHYA; COUTO DE AZEVEDO FERNANDES, NATALIA COELHO; ESPERON, FERNANDO; CATAO-DIAS, JOSE LUIZ; SACRISTAN, CARLOS. Adenovirus surveillance in wild carnivores from Brazil. INFECTION GENETICS AND EVOLUTION, v. 99, p. 7-pg., 2022-04-01. (16/20956-0, 18/25069-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

EWBANK, Ana Carolina. Identification and characterization of antimicrobial resistant genes in the microbiome of seabirds of the Brazilian coast. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.