Study of the development, stability and components o f a lignocellulose decongestan tbacterial consortium

• Grant number: 17/21667-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: June 01, 2018
• End date: November 30, 2019
• Field of knowledge: Agronomical Sciences - Agronomy
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Lúcia Maria Carareto Alves
• Grantee: Anna Carolina de Oliveira Souza
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The production the first-generation ethanol generates waste, including bagasse and sugarcane straw that can be used as raw material for the production the second-generation ethanol. The production of alcohol using lignocellulosic material is a process of sustainable use of various agroindustrial residues in addition to sugarcane bagasse. However, in order that this process happens in an efficient and economically feasible way, the biggest challenge in the production the second generation ethanol is to find the best option to transform hydrolysed cellulose and hemicellulose into hexose and pentoses that can be fermented. The aim of this work is to study the evolution of organisms that constitute a bacterial consortium with the capacity to use biomass as a source of carbon and to relate the organisms that constitute it with the capacity of deconstruction of the lignocellulolytic biomass and the liberation of fermentable sugars. The study of the succession of bacterial species in the consortium will be evaluated during 20 days from the genetic material and the sequence of the 16S rRNA gene obtained from cultures of the consortium amid lignocellulose fibers. Analysis of the composition of the fibrous fractions and the liberation of sugars and organic acids by HPLC chromatography of the liquid phase of the culture medium during the period studied will be performed. Thus, the relation between the bacterial population and the process efficiency can be evaluated so that, using fermenters with laboratory capacity, tests will be conducted to estimate the usefulness of the consortium for an application in industrial production. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Anna Carolina de Oliveira. Development, stability and component study of a deconstructing bacterial consortium of lignocellulose. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.