Studying the first cell communication signals between sugarcane and pathogens: focus on Reactive Oxygen Species.

• Grant number: 18/07615-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2018
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Silvana Aparecida Creste Dias de Souza
• Grantee: Andressa Peres Bini
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Campinas , SP, Brazil
• Associated research grant: 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case, AP.BIOEN.TEM

Abstract

Plant-pathogen interaction is a fundamental process that impacts agroecosystems. Understand the biological components and processes that control these interactions is expected to improve crop productivity. The thematic project: "Genes, Genomes & Transposable Elements contribution to plant-microbe interaction: a sugarcane study case" (FAPESP 2016/17545-8) aims to tackle this complex system using genome-wide molecular and bioinformatic tools to uncover key genes that control or mediate the interaction of sugarcane with three pathogens (Leifsonia xyli subsp. xyli; Xanthomonas albilineans; Sporisorium scitamineum). As part of the thematic project and to support the experimental designs proposed, this project have as main goals (i) the selection of sugarcane ROS genes and defense genes based in cell wall proteomics approach; (ii) the use of a real-time PCR assay to early detect and monitor the bacterial pathogens L. xyli subsp. xyli and X. albilineans and the sugarcane endosymbiont Gluconacetobacter diazotrophicus, to determine the time points to be used for the RNA capture experiments; (iii) validate sugarcane oxidative burst responses and pathogens protecting mechanisms against oxidative stress in sugarcane resistant and susceptible genotypes. We expect to identify key genes of the host defense machinery and microbial pathogenesis that could be used for screening resistant genotypes and to gain knowledge on plant-pathogen interactions. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PETERS, L. P.; TEIXEIRA-SILVA, N. S.; BINI, A. P.; SILVA, M. M. L.; MORAES, N.; CRESTANA, G. S.; CRESTE, S.; AZEVEDO, R. A.; CARVALHO, G.; MONTEIRO-VITORELLO, C. B.. Differential responses of genes and enzymes associated with ROS protective responses in the sugarcane smut fungus. FUNGAL BIOLOGY, v. 124, n. 12, p. 1039-1051, DEC 2020. (17/13268-2, 18/07615-4, 16/16376-8, 13/15014-7, 16/17545-8)

BINI, ANDRESSA PERES; ROSSI, GUILHERME DUARTE; POESCHL, YVONNE; SERRA, MARINA CARNAZ DUARTE; CAMARGO, LUIS EDUARDO ARANHA; MONTEIRO-VITORELLO, CLAUDIA BARROS; VAN SLUYS, MARIE-ANNE; DAM, NICOLE M. VAN; UTHE, HENRIETTE; CRESTE, SILVANA. Molecular, biochemical and metabolomics analyses reveal constitutive and pathogen-induced defense responses of two sugarcane contrasting genotypes against leaf scald disease. Plant Physiology and Biochemistry, v. 203, p. 14-pg., 2023-09-25. (18/07615-4, 18/21693-8, 16/17545-8)

BINI, ANDRESSA PERES; DUARTE SERRA, MARINA CARNAZ; PASTORE, IZADORA FARINA; BRONDI, CAROLINA VELUCI; ARANHA CAMARGO, LUIS EDUARDO; MONTEIRO-VITORELLO, CLAUDIA BARROS; VAN SLUYS, MARIE-ANNE; ROSSI, GUILHERME DUARTE; CRESTE, SILVANA. Transmission of Xanthomonas albilineans by the spittlebug, Mahanarva fimbriolata (Hemiptera: Cercopidae), in Brazil: first report of an insect vector for the causal agent of sugarcane leaf scald. JOURNAL OF INSECT SCIENCE, v. 23, n. 6, p. 8-pg., 2023-11-01. (18/07615-4, 18/21693-8, 16/17545-8)

ISHIDA, JULIANE K.; BINI, ANDRESSA P.; CRESTE, SILVANA; VAN SLUYS, MARIE-ANNE. Towards defining the core Saccharum microbiome: input from five genotypes. BMC Microbiology, v. 22, n. 1, p. 16-pg., 2022-08-08. (16/17545-8, 18/07615-4)

PERES BINI, A.; SERRA, M. C. D.; SILVA, H. R. V.; RAGAGNIN, G. T.; VITORELLO, C. B. M.; CAMARGO, L. E. A.; VAN SLUYS, M. A.; CRESTE, S.. Assessing early resistance responses of sugarcane to Xanthomonas albilineans. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 32, n. 10, p. 1-pg., 2019-10-01. (18/07615-4, 16/17545-8)