Neonatal anoxia corresponds to oxygen deprivation at birth, and is considered an important clinical problem that causes a great impact on public health by causing in those who survive severe and permanent sequelae. The hypoxic state triggers several cascades that lead to cell death in susceptible brain structures. In addition, it is suggested that neonatal oxygen deprivation may trigger changes in the expression of certain genes caused by epigenetic changes in both DNA and histones. In this project, we propose the evaluation of the effects of neonatal anoxia on the distribution and protein levels of the enzyme EZH2, an important histone-methyltransferase that plays a fundamental role in regulating gene expression indirectly, acting on histone modifications leading to greater or lesser expression of essential genes for cell maintenance and differentiation. For anoxia, animals on their first day of life (P1) will be exposed to 100% nitrogen gas at 37 ° C for 25 minutes, as previously described in the literature. EZH2 levels and their distribution will be analyzed 24 hours after neonatal anoxia in the hippocampus of the animals.
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