Solid-phase microextraction and gas chromatography in the lipidomic analysis of colorectal cancer patients' plasma

• Grant number: 17/25017-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2018
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Patrícia de Oliveira Carvalho
• Grantee: Gabrielle Kristine Doratiotto de Santis
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

Potential biomarkers of responses to alterations stemming from the development of cancer cells can be effectively evaluated by adopting lipidomic strategies using high resolution mass spectrometry and gas chromatography techniques for a more accurate identification and broad-scale quantification of alterations in cell and plasma lipids. This proposal to conduct a systematic qualitative and quantitative evaluation of total lipids and their fractions in blood samples (plasma and erythrocytes) drawn from volunteer colorectal cancer patients and healthy individuals, making use of solid phase micro extraction and gas chromatography is part of a larger, regular research project (FAPESP 2016/11905-2). The proposed study will contribute to lipidomic studies in cancer, to the endeavors to identify lipid biomarkers and to furthering understanding of the molecular bases of the pathology.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, ANNA MARIA A. P.; MESSIAS, MARCIA C. F.; DUARTE, GUSTAVO H. B.; DE SANTIS, GABRIELLE K. D.; MECATTI, GIOVANA C.; PORCARI, ANDREIA M.; MURGU, MICHAEL; SIMIONATO, ANA VALERIA C.; ROCHA, THALITA; MARTINEZ, CARLOS A. R.; et al. Plasma Lipid Profile Reveals Plasmalogens as Potential Biomarkers for Colon Cancer Screening. METABOLITES, v. 10, n. 6, JUN 2020. (17/25017-4, 18/13317-6)