Introduction: Usutu virus (USUV) belongs to the family Flaviviridae, genus Flavivirus and is responsible for the occurrence of human and veterinary encephalitis throughout Africa-Central Europe continents. Symptomatic USUV infections are characterized by a non-specific febrile illness similar to influenza, while severe manifestations result in neurological symptoms including encephalitis or meningoencephalitis. Guanosine is a neuroprotective compound tha have been used in the treatment of various neuropathologies. We believe that the beneficial properties of guanosine in the treatment of neurological diseases and its immunomodulatory effects may be beneficial in the context of the neurological disease caused by USUV. There are still no treatments or vaccines available against USUV. The study of viral biology and pathogenic potential is needed. Objective: develop an experimental model of infection with USUV in mice to study the disease and to test protective properties of guanosine. Preliminary Results: The proponent already has cell lines and USUV, produced viral stocks and standardized the plate titration technique to measure USUV in varied biological samples. Initial experiments indicate that USUV causes disease and mortality in adult mice in an inoculum-dependent manner. Therefore, these results indicates that the establishment of an animal model for guanosine testing is a viable strategy.
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