Physiological function and origin of the spherulocytes in the sea urchin Paracentrotus lividus (Echinodermata: Echinoidea)

• Grant number: 18/14497-8
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 20, 2018
• End date: July 14, 2019
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Márcio Reis Custódio
• Grantee: Vinicius Queiroz Araújo
• Supervisor: Vincenzo Arizza
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Università degli Studi di Palermo (UNIPA), Italy
• Associated to the scholarship: 15/21460-5 - Physiological function, chemical composition and ontogeny of Echinoid spherulocytes (Echinodermata)., BP.DR

Abstract

In vertebrates, blood cells accomplish many important physiological and immune functions. In these animals, especially in mammals, the level of knowledge is high and the basic aspects have already been known. In relation to invertebrates the situation is different. For these organisms, the tissue corresponding to blood is the coelomic fluid or haemocell (depending of the group), whose cells (celomocytes or haemocytes) perform related functions. However, very little is known about the specific roles of these cells. In echinoderms, only the phagocyte has been deeply studied and other cell types, such as spherulocytes, have been the subject of less work, even in the most studied echinoderm models. Thus, the present project aims to investigate the physiological functions of the spherulocytes in the Mediterranean sea urchin Paracentrotus lividus. This species will be used, whose subpopulations of spherulocytes will be identified and characterized. In addition, experiments will be carried out to ascertain the probable physiological roles and determine the possible sites of origin of these celomocytes. To achieve these objectives, procedures such as histology, electron microscopy, immunohistochemistry and larval culture will be performed. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; ROZAS, ENRIQUE E.; CUSTODIO, MARCIO R.. Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy. Revista de Biología Tropical, v. 69, n. 1, p. 171-184, 2021. (13/50218-2, 18/14497-8, 15/21460-5)

QUEIROZ, VINICIUS; MUXEL, SANDRA M.; INGUGLIA, LUIGI; CHIARAMONTE, MARCO; CUSTODIO, MARCIO R.. Comparative study of coelomocytes from Arbacia lixula and Lythechinus variegatus: Cell characterization and in vivo evidence of the physiological function of vibratile cells. FISH & SHELLFISH IMMUNOLOGY, v. 110, p. 1-9, MAR 2021. (18/14497-8, 15/21460-5)

NOGUEIRA, MARCOS M.; NEVES, ELIZABETH; QUEIROZ, VINICIUS; JOHNSSON, RODRIGO. Does coral morpholoy influence the richness and abundance of associated ophiuroids? An evaluation of differences in habitat structure provided by Mussismilia corals. CAHIERS DE BIOLOGIE MARINE, v. 61, n. 2, p. 219-227, 2020. (18/14497-8, 15/21460-5)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; ROZAS, ENRIQUE E.; CUSTODIO, MARCIO R.. Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy. Revista de Biología Tropical, v. 69, p. 14-pg., 2021-03-01. (15/21460-5, 18/14497-8, 13/50218-2)

QUEIROZ, VINICIUS; MAURO, MANUELA; ARIZZA, VINCENZO; CUSTODIO, MARCIO R.; VAZZANA, MIRELLA. The use of an integrative approach to identify coelomocytes in three species of the genus Holothuria (Echinodermata). Invertebrate Biology, DEC 2021. (15/21460-5, 18/14497-8)

SALES, LICIA; QUEIROZ, VINICIUS. Sexual dimorphism in the parasitic snail Nanobalcis worsfoldi: a histological and morphometric approach with insights for the family Eulimidae. CANADIAN JOURNAL OF ZOOLOGY, v. 99, n. 11, p. 995-1001, NOV 2021. (15/21460-5, 18/14497-8, 13/08425-0)

MAURO, MANUELA; QUEIROZ, VINICIUS; ARIZZA, VINCENZO; CAMPOBELLO, DANIELA; CUSTODIO, MARCIO REIS; CHIARAMONTE, MARCO; VAZZANA, MIRELLA. Humoral responses during wound healing in Holothuria tubulosa (Gmelin, 1788). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 253, APR-MAY 2021. (15/21460-5, 18/14497-8)

SALES, LICIA; QUEIROZ, VINICIUS; LUCENA, RUDA A.; MIGOTO, ALVARO E.. An unprecedented observation of the nudibranch Spurilla braziliana (Gastropoda: Aeolidiidae) as a food resource of the pycnogonid Pigrogromitus timsanus (Arthropoda: Ascorhynchoidea). NAUTILUS, v. 135, n. 1, p. 32-34, MAY 13 2021. (15/21460-5, 18/14497-8)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; CUSTODIO, MARCIO R.. Comparative evaluation of coelomocytes in Paracentrotus sea urchins: Description of new cell types and insights on spherulocyte maturation and sea urchin physiology. ZOOLOGISCHER ANZEIGER, v. 300, p. 14-pg., 2022-07-14. (18/14497-8, 15/21460-5, 21/10161-8)

QUEIROZ, VINICIUS; SALES, LICIA; NEVES, ELIZABETH; JOHNSSON, RODRIGO. Siderastrea stellata Verrill, 1868 as a new host for the corallivore snail Coralliophila caribaea Abbott, 1958 and some biological aspects of their association. SPIXIANA, v. 44, n. 1, p. 2-pg., 2021-11-01. (13/08425-0, 18/14497-8, 15/21460-5)

CHIARAMONTE, MARCO; ARIZZA, VINCENZO; LA ROSA, SIMONA; QUEIROZ, VINICIUS; MAURO, MANUELA; VAZZANA, MIRELLA; INGUGLIA, LUIGI. Allograft Inflammatory Factor AIF-1: early immune response in the Mediterranean sea urchin Paracentrotus lividus. ZOOLOGY, v. 142, OCT 2020. (15/21460-5, 18/14497-8)

SALES, LICIA; SANTOS, HENRIQUE B.; LIMA, SILVIO F. B.; QUEIROZ, VINICIUS. A new symbiont for the sea anemone Homostichanthus duerdeni: the spotted cleaner shrimp Periclimenes yucatanicus (Cnidaria, Anthozoa and Crustacea, Decapoda). SPIXIANA, v. 45, n. 1, p. 3-pg., 2022-11-01. (15/21460-5, 18/14497-8, 13/08425-0)

QUEIROZ, VINICIUS. An unprecedented association of an encrusting bryozoan on the test of a live sea urchin: epibiotic relationship and physiological responses. MARINE BIODIVERSITY, v. 50, n. 5, p. 7-pg., 2020-09-18. (15/21460-5, 18/14497-8)