Methylome and transcriptome analysis of chickens subjected to illumination stress in the livestock production environment

• Grant number: 18/13600-0
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: January 16, 2019
• End date: January 15, 2020
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Luiz Lehmann Coutinho
• Grantee: Fábio Pértille
• Supervisor: Carlos Guerrero Bosagna
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Institution abroad: Linköping University (LiU), Sweden
• Associated to the scholarship: 16/20440-3 - Epigenetic analysis of methylation profile of chickens submitted to different stress conditions in the livestock production environment, BP.PD

Abstract

Stress in production animals generated by common production practices is a frequent issue of concern. Besides the ethical issue of inducing stress in animals, some practices in animal industry may have consequences from a human health perspective. The environment where production animals are raised is fundamental to determine not only their later health and wellbeing, but also the quality of the food originating from them. Stress conditions to which animals are subjected include extreme illumination patterns, social isolation, rough handling and under- or overfeeding. In animals undergoing distress, hormonal responses are produced. These include variations in testosterone, epinephrine, prolactin and cortisol. It is expected that if animals are constantly subjected to stress and systemic hormonal changes, this exposure will affect the epigenome of a variety of cell types including mitochondria and, consequently, the gene expression. Moreover, mitochondrial epigenetic modifications (MEMs) represent an alternative point of control mediating the changes in mitochondrial form and function that are observed in response to environmental stressors and disease. However, few studies have quantified MEMs resulting from environmental stress and none has correlated them to mitochondrial or organismal phenotypes. In order to determine whether the effects of long-term (stressful) rearing conditions of chickens can produce long-term epigenomic effects in both autosomal and mitochondrial epigenomes, we will investigate transcriptional and epigenetic effects of exposure to unpredictable light patterns. This painless stress is commonly used to alter growth rate and to allow birds to achieve maximal mass accretion. The pineal gland is a region of vertebrate brains that regulates the circadian rhythm of individuals through the secretion of melatonin according to the patterns of light they experience. These mechanisms require gene activation dependent upon light exposures, which strongly suggests involvement of epigenetic mechanisms regulating gene transcription. The present study will focus on whether long-term gene expression and epigenetic changes can be produced in the pineal gland of chickens after early life exposure to stressful illuminations patterns. The results of this research will have an impact not only from the animal welfare viewpoint. They will serve as a basis for the use of epigenetic tools to determine the stress exposure during animal's life and may reveal new epigenetic mechanisms related to human health.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUNDMAN, ANN-SOFIE; PERTILLE, FABIO; LEHMANN COUTINHO, LUIZ; JAZIN, ELENA; GUERRERO-BOSAGNA, CARLOS; JENSEN, PER. DNA methylation in canine brains is related to domestication and dog-breed formation. PLoS One, v. 15, n. 10, OCT 29 2020. (16/20440-3, 18/13600-0)

PERTILLE, FABIO; IBELLI, ADRIANA MERCIA GUARATINI; SHARIF, MAJ EL; POLETI, MIRELE DAIANA; FROHLICH, ANNA SOPHIE; REZAEI, SHIVA; LEDUR, MONICA CORREA; JENSEN, PER; GUERRERO-BOSAGNA, CARLOS; COUTINHO, LUIZ LEHMANN. Putative Epigenetic Biomarkers of Stress in Red Blood Cells of Chickens Reared Across Different Biomes. FRONTIERS IN GENETICS, v. 11, NOV 2 2020. (16/20440-3, 18/13600-0, 14/08704-0)

DA SILVA, ARTHUR NERY; ARAUJO, MICHELLE SILVA; PERTILLE, FABIO; ZANELLA, ADROALDO JOSE. How Epigenetics Can Enhance Pig Welfare?. ANIMALS, v. 12, n. 1, JAN 2022. (16/20440-3, 18/13600-0)

PERTILLE, FABIO; DA SILVA, VINICIUS H.; JOHANSSON, ANNA M.; LINDSTROM, TOM; WRIGHT, DOMINIC; COUTINHO, LUIZ L.; JENSEN, PER; GUERRERO-BOSAGNA, CARLOS. Mutation dynamics of CpG dinucleotides during a recent event of vertebrate diversification. Epigenetics, v. 14, n. 7, MAY 2019. (16/20440-3, 14/08704-0, 18/13600-0)

TATEMOTO, PATRICIA; PERTILLE, FABIO; BERNARDINO, THIAGO; ZANELLA, RICARDO; GUERRERO-BOSAGNA, CARLOS; ZANELLA, ADROALDO JOSE. An enriched maternal environment and stereotypies of sows differentially affect the neuro-epigenome of brain regions related to emotionality in their piglets. Epigenetics, v. 18, n. 1, p. 26-pg., 2023-12-31. (16/20440-3, 18/13600-0, 17/05604-2)

RODRIGUEZ, EMILIANO A. VIDELA; PERTILLE, FABIO; GUERRERO-BOSAGNA, CARLOS; MITCHELL, JOHN B. O.; JENSEN, PER; SMITH, V. ANNE. Practical application of a Bayesian network approach to poultry epigenetics and stress. BMC Bioinformatics, v. 23, n. 1, p. 16-pg., 2022-07-01. (18/13600-0, 16/20440-3)

PERTILLE, FABIO; ALVAREZ-RODRIGUEZ, MANUEL; DA SILVA, ARTHUR NERY; BARRANCO, ISABEL; ROCA, JORDI; GUERRERO-BOSAGNA, CARLOS; RODRIGUEZ-MARTINEZ, HERIBERTO. Sperm Methylome Profiling Can Discern Fertility Levels in the Porcine Biomedical Model. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 5, MAR 2021. (16/20440-3, 18/13600-0)

GUERRERO-BOSAGNA, CARLOS; PERTILLE, FABIO; GOMEZ, YAMENAH; REZAEI, SHIVA; GEBHARDT-HENRICH, SABINE G.; VOGELI, SABINE; STRATMANN, ARIANE; VOELKL, BERNHARD; TOSCANO, MICHAEL J.. DNA methylation variation in the brain of laying hens in relation to differential behavioral patterns. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 35, SEP 2020. (16/20440-3, 18/13600-0)