Dental pulp stem cell biology

• Grant number: 18/13675-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2018
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Supervisor: Jacques Eduardo Nor
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University of Michigan, United States
• Associated to the scholarship: 17/11396-3 - Protein gene expression by stem cells from pulp of deciduous teeth after the use of low intensity laser, BP.DR

Abstract

This study aims to evaluate the viability and proliferation of Stem Cell from Human Exfoliated Teeth (SHED); mRNA gene expression and protein synthesis of DMP-1, DSPP, VEGF, and FGF2 after photobiomodulation; and SHED differentiation into endothelial cells through VEGF expression. The characterization of the SHED will be performed with hMSC Analysis Kit with flow cytometry. The groups will be divided according to the energy densities. The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR, ELISA, Immunofluorescence, Western Blot, Capillary Sprouting Assay, Tooth Slices/Scaffold, and RGB Assay. The values in triplicate will be analysed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test respecting normal and non-normal distribution, respectively. The level of significance of 5% will be adopted for statistically significant differences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERGAMO, M. T.; ZHANG, Z.; OLIVEIRA, T. M.; NOR, J. E.. VEGFR1PRIMES A UNIQUE COHORT OF DENTAL PULP STEM CELLS FOR VASCULOGENIC DIFFERENTIATION. EUROPEAN CELLS & MATERIALS, v. 41, p. 332-344, JAN-JUN 2021. (18/13675-0)