Manufacturing and characterization of nanofibers de ALGINATE/SILK fibroin incorporated with pequi oil applied in the treatment of skin wounds

• Grant number: 17/26194-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2018
• End date: September 30, 2020
• Field of knowledge: Engineering - Materials and Metallurgical Engineering
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcia Cristina Branciforti
• Grantee: Francisco Vieira dos Santos
• Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

In Brazil, skin lesions are a serious public health problem with regard to the high number of patients with some type of skin lesion. The large number of people with some type of ulcer or burn contributes to raising public spending on health, and also causes irreversible damage to the quality of life of the population. In this project, it is desired to use the knowledge of tissue engineering to produce bioactive electrospun blankets using two natural biopolymers (alginate and silk fibroin) in the form of a cutaneous dressing. However, silk fibroin when used in isolation in the preparation of a dressing has low physical-chemical and mechanical characteristics. In this context, alginate appears as a suitable candidate in the improvement of the swelling properties, mechanical resistance, permeability to water vapor and oxygen, hydrophilicity, fluid absorption and hemostatic capacity of the dressing. In addition, the knowledge of the chemical structures and the morphology of the blends, as well as the hydrophilic and hydrophobic characteristics of these compounds allows to obtain a cutaneous dressing with synergy in the physical, chemical, mechanical, thermal and biological properties. Regarding the antiinflammatory and healing properties, the current project proposes the incorporation of pequi oil in combination with the two natural polymers associated with the electrospinning technique to obtain cutaneous dressings that can be applied in the treatment of wounds. To date, little is known about the properties of alginate blends and silk fibroin incorporated with pequi oil produced by electrospinning, and such a study will certainly bring positive scientific and technological impacts. In view of this scenario, electrospun blankets of alginate / silk fibroin blend incorporated with pequi oil, present as promising low-cost cutaneous dressings with high technological performance and that can be applied in the treatment of wounds and burns.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, FRANCISCO VIEIRA; YOSHIOKA, SERGIO AKINOBU; BRANCIFORTI, MARCIA CRISTINA. Large-area thin films of silk fibroin prepared by two methods with formic acid as solvent and glycerol as plasticizer. Journal of Applied Polymer Science, v. 138, n. 31, p. 14-pg., 2021-03-27. (17/26194-7)

DOS SANTOS, FRANCISCO VIEIRA; YOSHIOKA, SERGIO AKINOBU; BRANCIFORTI, MARCIA CRISTINA. Large-area thin films of silk fibroin prepared by two methods with formic acid as solvent and glycerol as plasticizer. Journal of Applied Polymer Science, MAR 2021. (17/26194-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Francisco Vieira dos. Development and characterization of silk fibroin thin films aiming biomedical applications. 2022. Master's Dissertation - Universidade de São Paulo (USP). Escola de Engenharia de São Carlos (EESC/SBD) São Carlos.