Study of the release of ATP in the dorsal root ganglion in the development of inflammatory hyperalgesia

• Grant number: 17/19105-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2018
• Effective date (End): August 31, 2021
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Carlos Amilcar Parada
• Grantee: Júlia Borges Paes Lemes
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 19/15255-0 - Pannexin 1 channels and ATP signaling in mechanisms of peripheral pain sensitization, BE.EP.DR

Abstract

ATP (adenosine triphosphate), initially known as an intracellular energy source, plays an important role in intercellular communication in physiological and pathological conditions. This molecule appears to be important in the development of inflammatory hyperalgesia in the peripheral nervous system and is considered an important mediator in the communication between primary afferent neurons and satellite cells in the sensory ganglia. ATP can be released by the body of sensory neurons of the dorsal root ganglion (GRD), being stored in lysosomal vesicles and later released by exocytosis. Another form of release of ATP is through membrane channels called Panexin (panx). Recent research shows that pannexin (panx1) is present in neurons and satellite cells of the GRD and that this channel is important in the development of neuropathic pain. Previous results from the group suggest that the ATP released in the GRD acts on P2X7 receptors in satellite glial cells and that these cells participate in the processing of inflammatory pain. Although a relationship between the purinergic receptor P2X7 and panx1 has been observed in the central nervous system, there is no data yet on this relationship in the DRG. Considering that release of ATP in DRG may be a mechanism in the development of inflammatory hyperalgesia, the aim of this project is to study the mechanisms involved in the release of ATP in the DRG during the development of carrageenan-induced hyperalgesia and in the capsaicin-induced nociception in the peripheral tissue Of mice.Considering the importance of the mechanisms involving ATP in the communication between primary nociceptive neurons and DRG satellite cells, this study may provide subsidies for the establishment of new pharmacological targets in the control of inflammatory pain.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LEMES, Júlia Borges Paes. Study of ATP release mediated by Panx1 and exocytosis on dorsal root ganglion: effect on nociception and hyperalgesia. 2021. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.