Study of post-transcriptional gene regulation processes in the somatic embryogenesis in Araucaria angustifolia

• Grant number: 18/08215-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2018
• End date: February 28, 2022
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Eny Iochevet Segal Floh
• Grantee: Leandro Francisco de Oliveira
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Post-transcriptional gene regulation occurs by different mechanisms, one of them is the regulation by small RNAs (sRNAs), which are regulatory elements non-coding. In plants, among different classes of sRNAs, micro-RNAs (miRNAs) are the most studied in various development processes, including embryogenesis. However, in gymnosperms, the functions of specifics miRNAs in the regulation of genes during somatic embryogenesis were not totally elucidated. In Araucaria angustifolia, an endangered native conifer with economic importance, studies performed in embryogenic cultures using platforms of transcripts and proteome have been revealed the participation of post-transcriptional regulation mechanisms. Comparative studies between zygotic and somatic embryogenesis, and between two cell lines with distinct embryogenic potential, have been used for a better comprehension of physiological, biochemical and molecular aspects of embryogenesis. The present project aims to comprehend the mechanisms of post-transcriptional gene regulation and their correlation with somatic embryogenesis process. For this, will be performed an analysis of sRNAs profile, as well as the identification of miRNAs and their target genes, in two cell lines of A. angustifolia, with distinct embryogenic potential. The obtained results will be evaluated considering the transcriptome and proteome platforms of A. angustifolia, built in the BIOCEL. This propose will introduce new approaches in the studies of the mechanisms that regulate the embryogenesis in gymnosperms, specifically in A. angustifolia, and may also to elucidate new aspects related to post-transcriptional regulation in this plant system and, finally, will help the optimization of in vitro culture conditions for the somatic embryogenesis.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA PRIM, PAMELA; DE OLIVEIRA, LEANDRO FRANCISCO; RIBAS, LUCIANA LOPES FORTES. An efficient protocol for in vitro propagation of Trichilia pallida Swartz (Meliaceae): a potential tree for natural insecticide production. PLANT CELL TISSUE AND ORGAN CULTURE, v. 144, n. 2, p. 469-476, FEB 2021. (18/08215-0)

DE OLIVEIRA, LEANDRO FRANCISCO; PIOVEZANI, AMANDA RUSISKA; IVANOV, DIMITRE A.; YOSHIDA, LEONARDO; SEGAL FLOH, ENY IOCHEVET; KATO, MASSUO JORGE. Selection and validation of reference genes for measuring gene expression in Piper species at different life stages using RT-qPCR analysis. Plant Physiology and Biochemistry, v. 171, p. 12-pg., 2022-01-07. (18/08215-0, 15/26961-2, 20/03791-2)

ELBL, PAULA M.; DE SOUZA, DIEGO T.; ROSADO, DANIELE; DE OLIVEIRA, LEANDRO F.; NAVARRO, V, BRUNO; MATIOLI, SERGIO R.; FLOH, ENY I. S.. Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pin. Gene, v. 817, APR 5 2022. (18/08215-0, 16/17541-2, 17/25680-5)

DE OLIVEIRA, LEANDRO FRANCISCO; DOS SANTOS, ANDRE LUIS WENDT; FLOH, ENY IOCHEVET SEGAL. Polyamine and amino acid profiles in immature Araucaria angustifolia seeds and their association with embryogenic culture establishment. TREES-STRUCTURE AND FUNCTION, v. 34, n. 3, DEC 2019. (15/21075-4, 12/22738-9, 16/17541-2, 17/01284-3, 18/08215-0)