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Evaluation of the effect of antipsychotics and the cannabidiol in an oligodendrocytes culture treated with cuprizone: implications for myelination

Grant number: 18/10362-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2018
Effective date (End): July 31, 2020
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Daniel Martins-de-Souza
Grantee:Ana Caroline Brambilla Falvella
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/08711-3 - Developing a predictive test for a successful medication response and understanding the molecular bases of schizophrenia through proteomics, AP.JP


Disturbances of oligodendrocytes and myelin integrity are observed in certain disorders, such as schizophrenia. In this context, toxic models of demyelination are used to understand the mechanisms involved in demyelination, remyelination and oligodendrocyte alterations. On the other hand, proteomic approaches are important for the understanding of processes involved in neurological disorders, through the elucidation of biochemical bases, and identification of differentially expressed pathways and proteins, in certain cerebral disorders. In the present work, the cuprizone-induced demyelination model will be used in human oligodendrocyte cell cultures (lineage MO3.13) to test the effects of antipsychotics (clozapine and haloperidol), cannabidiol and benztropine, in terms of their potential neuroprotective effects. After the treatments mentioned above, the proteome will be extracted and digested for analysis by nano-chromatography coupled to mass spectrometry. Thereby, this project aims to investigate the proteins and pathways involved in the demyelination, remyelination and proliferation of oligodendrocytes, in schizophrenia model. In this context, the present study will contribute to the understanding of the molecular biochemical mechanisms involved in the action of these drugs on oligodendrocytes, and their possible implications on the pathophysiology and treatment of schizophrenia.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FALVELLA, ANA CAROLINE BRAMBILLA; SMITH, BRADLEY JOSEPH; SILVA-COSTA, LICIA C.; VALENCA, ALINE G. F.; CRUNFLI, FERNANDA; ZUARDI, ANTONIO W.; HALLAK, JAIME E.; CRIPPA, JOSE A.; ALMEIDA, VALERIA DE; MARTINS-DE-SOUZA, DANIEL. Cannabidiol Displays Proteomic Similarities to Antipsychotics in Cuprizone-Exposed Human Oligodendrocytic Cell Line MO3.13. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 14, . (18/03422-7, 08/09009-2, 17/18242-1, 19/00098-7, 17/25588-1, 19/22398-2, 18/10362-0, 18/03450-0, 18/03673-0)
GOUVEA-JUNQUEIRA, DANIELLE; FALVELLA, ANA CAROLINE BRAMBILLA; ANTUNES, ANDRE SARAIVA LEAO MARCELO; SEABRA, GABRIELA; BRANDAO-TELES, CAROLINE; MARTINS-DE-SOUZA, DANIEL; CRUNFLI, FERNANDA. Novel Treatment Strategies Targeting Myelin and Oligodendrocyte Dysfunction in Schizophrenia. FRONTIERS IN PSYCHIATRY, v. 11, . (18/10362-0, 17/25588-1, 17/25055-3, 19/22398-2, 18/25439-9, 18/03673-0)
SEABRA, GABRIELA; FALVELLA, ANA CAROLINE B.; GUEST, PAUL C.; MARTINS-DE-SOUZA, DANIEL; DE ALMEIDA, VALERIA. Proteomics and Lipidomics in the Elucidation of Endocannabinoid Signaling in Healthy and Schizophrenia Brains. PROTEOMICS, v. 18, n. 18, SI, . (17/18242-1, 18/03673-0, 18/10362-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FALVELLA, Ana Caroline Brambilla. Antipsychotics and cannabidiol effects in cuprizone-treated oligodendrocytes: implications for myelination. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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