Dereplication profile of Nectandra oppositifolia Nees & Mart. (Lauraceae): identification and molecular characterization of antiparasitic metabolites

• Grant number: 18/18975-1
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: April 01, 2019
• End date: September 30, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: João Henrique Ghilardi Lago
• Grantee: Geanne Alexsandra Alves Conserva
• Supervisor: Emerson Ferreira Queiroz
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Institution abroad: Université de Genève, Switzerland
• Associated to the scholarship: 16/20633-6 - Bioactive metabolites from Nectandra oppositifolia Nees & Mart. (Lauraceae): molecular characterization, in vitro and in vivo antiparasitic potential evaluation and determination of mechanism of action, BP.DR

Abstract

Brazilian Atlantic forest is one of most important regions worldwide, considered as a biodiversity hotspot due to its high degree of biodiversity (plant, animals and microorganisms). Based in this aspect, the investigation of metabolites from different plants species from this biome could be a source of new prototypesto therapeutic treatment for several diseases, including neglected tropical diseases, such as malaria, leishmaniosis and Chagas Disease. As part of our continuous studies with Atlantic forest plant species with antiparasital activity, was selected Nectandra oppositifolia, which could be considered unkwonledge in chemicaland pharmacological point of view. To conduct this study (project FAPESP 2016/20633-6), the hexane and EtOH extracts from leaves and twigs of were prepared and displayed expressive antiparasitic potential against Trypanosoma cruzi and Leishmania infantum. The hexane extract from twigs was subjected to a bioassay-guided fractionation to afford three bioactive butenolides: isolinderanolide D, isolinderanolide E and secosubamolide A. Furthermore, dereplication procedures indicated the predocimance of licarin A from hexane extract from leaves of this specie. In a second part of this work dereplication strategies will be conducted to investigate the metabolic composition of the high bioactive EtOH extracts from twigs and leaves of N. oppositifolia at the Laboratory of Phytochemistry and Bioactive Natural Products of University of Geneva, in collaboration to Prof. Dr. Jean-Luc Wolfender and Emerson F. Queiroz. This procedure involve hyphenated techniques (LC/DAD, LC/MS and LC/NMR) and several chromatographic procedures aiming isolation of metabolites of N. oppositifolia. After isolation and identification of these metabolites, they will be subjected to antiparasitic evaluation as proposed in the project FAPESP 2016/20633-6. Thus, the period of six months in the Laboratory of Phytochemistry and Bioactive Natural Products of University of Geneva would be crucial to complete the investigation of bioactive extracts from leaves and twigs of the N. oppositifolia. Additonally, it will be an excellent opportunity to know different hyphenated analytical methods used to dereplication of natural products. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSERVA, GEANNE A.; COSTA-SILVA, THAIS A.; QUIROS-GUERRERO, LUIS M.; MARCOURT, LAURENCE; WOLFENDER, JEAN-LUC; QUEIROZ, EMERSON F.; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.. Kaempferol-3-O-alpha-(3,4-di-E-p-coumaroyl)-rhamnopyranoside from Nectandra oppositifolia releases Ca2+from intracellular pools of Trypanosoma cruzi affecting the bioenergetics system. Chemico-Biological Interactions, v. 349, NOV 1 2021. (16/20633-6, 21/04464-8, 21/02789-7, 18/18975-1)

ALVES CONSERVA, GEANNE A.; QUIROS-GUERRERO, LUIS M.; COSTA-SILVA, THAIS A.; MARCOURT, LAURENCE; PINTO, ERIKA G.; TEMPONE, ANDRE G.; FERNANDES, JOAO PAULO S.; WOLFENDER, JEAN-LUC; QUEIROZ, EMERSON F.; LAGO, JOAO HENRIQUE G.. Metabolite profile of Nectandra oppositifolia Nees & Mart. and assessment of antitrypanosomal activity of bioactive compounds through efficiency analyses. PLoS One, v. 16, n. 2, FEB 25 2021. (18/18975-1, 18/07885-1, 16/20633-6, 18/10279-6, 15/23403-9)

CONSERVA, G. A. A.; MORAIS, T. R.; RODRIGUES, V. C.; DE MORAES, J.; LAGO, J. H. G.. In vivo anthelmintic activity evaluation of licarin A, a neolignan isolated from leaves of Nectandra oppositifolia (Lauraceae). Planta Medica, v. 85, n. 18, p. 2-pg., 2019-12-01. (16/20633-6, 18/18975-1)