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In vitro analysis of the effectiveness of different dental bleaching protocols using violet led

Grant number: 18/15590-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2018
Effective date (End): October 31, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Patricia Moreira de Freitas Costa e Silva
Grantee:Bruno Bachiega da Silva
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The search for tooth whitening has grown all over the world, but the active bleaching agent, while effective, can be aggressive to the dental element. In this way, new techniques, such as the one that advocates the use of violet LED light, have arisen and need studies. In order to analyze and compare the effectiveness of violet LED light (405 - 410 nm) isolated or associated with hydrogen peroxide, this study will investigate 6 different tooth whitening techniques (n = 10) in bovine teeth previously pigmented intrinsically: G1 - Peroxide of 35% hydrogen (1x per week for 4 weeks 45 minutes of gel application); G2 - 35% hydrogen peroxide (1x per week, for 4 weeks, 10 minutes gel application); G3 - violet LED (1x per week, for 4 weeks); G4- violet LED (2x per week, for 2 weeks); G5 - violet LED (4x per week, for 1 week);G6 - violet LED + 35% hydrogen peroxide (hybrid technique, 1x / week, for 4 weeks, 10 minutes of gel application). The pigmented specimens will be submitted to color evaluation at predetermined times, using VITA Easy shade 3D Master spectrophotometer and Konica Minolta® spectrophotometer. In addition, two teeth of each group will have their surface analyzed, in a qualitative way, in Scanning Electron Microscope (SEM). The data of the color change test will be analyzed using Minitab® software, considering a 5% level of significance.

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