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Expression of proteins involved in mitophagy and retrograde traffic of autophagosomes prior and after protein aggregation associated with Alzheimer's Disease

Grant number: 18/11946-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2018
Effective date (End): September 30, 2019
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Merari de Fátima Ramires Ferrari
Grantee:Alan Moreira Henrique
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

It is believed that the loss of the protein quality control and the impairment of the autophagic pathway contributes to the cellular events that culminate in neurodegeneration. In view of this, the purpose of the present study is to evaluate the cellular quality control system in genetically modified mouse model for Down syndrome, which can also be used to study the Alzheimer's disease related to triplicated copies of important genes to this neurodegenerative disease. The study will be conducted before and after the appearance of the proteic aggregates that are characteristic of neurodegenerative diseases. To this end, levels of proteins associated with activation of autophagy will be analyzed (including mitophagy) in the hippocampus and locus coeruleus of 2- and 5-month old mice. The Project will include the use of genotyping techniques (PCR) to determine which animals will be used and Western Blot to evaluate the proteins of interest.