Aqueous two-phase micellar systems based on ionic liquids as platforms for the curcumin encapsulation in polymeric micelles

• Grant number: 18/05111-9
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2018
• Effective date (End): December 31, 2019
• Field of knowledge: Engineering - Chemical Engineering - Chemical Technology
• Principal researcher: Jorge Pereira
• Grantee: Isabelle Souza Kurnik
• Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 19/05624-9 - Encapsulation and in vitro release studies of curcumin in polymeric micelles composed of PEO-PPO-PEO triblock copolymers, BE.EP.IC

Abstract

Polymer micelles (MP) are described as excellent carriers of drugs with low solubility in the aqueous environment, such as curcumin (CCM), an important antitumoral, but despite this, these nanostructures are mostly obtained from methods that use considerable fractions of organic solvents. Thus, considering the concerns related to the high toxicity and flammability, denaturing effect and the solvents' disposal requirements, it is of utmost importance in the nanobiotechnological field to develop an innovative and alternative aqueous formulation for delivering lipophilic drugs with poor water solubility. Accordingly, we believe that aqueous two-phase micellar systems (ATPMS) can be implemented as a new platform for obtaining MP using a more biocompatible process (i.e., without adding solvents), fast and efficient forCCM encapsulation. To achieve this objective, the present project aims to determine the binodal curves for systems containing the copolymer Pluronic L35 in phosphate buffer (pH7.4) in the absence and presence of ionic liquids (ILs) as adjuvants. In addition, a second step will evaluate the ability of Pluronic L35/phosphate buffer + ILs in CCM encapsulation, using different temperatures and concentrations of L35, determining the rates of encapsulation and loading the drug in the MP. Finally, it is intended to characterize the nanostructures and to study the stability and the in vitro release profile of CCM from the micellar aggregates at different temperatures and over time.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KURNIK, ISABELLE S.; D'ANGELO, NATALIA A.; MAZZOLA, PRISCILA G.; CHORILLI, MARLUS; KAMEI, DANIEL T.; PEREIRA, JORGE F. B.; VICENTE, ANTONIO A.; LOPES, ANDRE M. Polymeric micelles using cholinium-based ionic liquids for the encapsulation and release of hydrophobic drug molecules. BIOMATERIALS SCIENCE, v. 9, n. 6, p. 2183-2196, MAR 21 2021. Web of Science Citations: 0.

KURNIK, ISABELLE S.; MUSSAGY, CASSAMO U.; PEREIRA, JORGE F. B.; LOPES, ANDRE M. Amphiphilic copolymer aqueous solutions with cholinium ionic liquids as adjuvants: New insights into determination of binodal curves and phase-separation mechanisms. JOURNAL OF MOLECULAR LIQUIDS, v. 318, NOV 15 2020. Web of Science Citations: 0.

KURNIK, ISABELLE S.; NORONHA, MARIANA A.; CAMARA, MAYRA C. C.; MAZZOLA, PRISCILA G.; VICENTE, ANTONIO A.; PEREIRA, JORGE F. B.; LOPES, ANDRE M. Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids. Separation and Purification Technology, v. 250, NOV 1 2020. Web of Science Citations: 0.