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Determination of neuronal function and oxidative metabolic profile in free-floating adult human brain-derived slice cultures

Grant number: 18/06614-4
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2018
Effective date (End): January 31, 2020
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Adriano Silva Sebollela
Grantee:Glaucia Maria de Almeida
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Adult human brain organotypic cultures are a powerful method for studying cellular and molecular aspects of neuropathologies, as well as the effects of neuroprotective drugs. The main advantages of this method include maintaining the brain structure and its cellular connectivity. However, there is still a lack of studies that better characterize this model, not only to obtain more reliable results, but also to expand its applications. The objective this present project is to expand the characterization of a model of adult human brain using cortical slices obtained from patients undergoing surgery for epilepsy, observing the oxidative metabolism of this tissue by respirometry and citrate synthase activity. We will also assess the maintenance of neuronal function over the days in culture by assessing c-fos protein levels. Furthermore, we will apply our model in studies of infection of the central nervous system by Oropouche virus, determining infected neural phenotypes, apoptotic markers and possible cellular parameters that can be altered as a result this infection. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERNANDES, ARTUR; MENDES, NIELE DIAS; ALMEIDA, GLAUCIA MARIA; NOGUEIRA, GIOVANNA ORLOVSKI; MACHADO, CARLA DE MORAES; DE CASTRO HORTA-JUNIOR, JOSE DE ANCHIETA; ASSIRATI JUNIOR, JOAO ALBERTO; GARCIA-CAIRASCO, NORBERTO; NEDER, LUCIANO; SEBOLLELA, ADRIANO. Short-Term Free-Floating Slice Cultures from the Adult Human Brain. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 153 NOV 2019. Web of Science Citations: 0.

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