Determination of neuronal function and oxidative metabolic profile in free-floating adult human brain-derived slice cultures

• Grant number: 18/06614-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2018
• End date: January 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Adriano Silva Sebollela
• Grantee: Glaucia Maria de Almeida
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Adult human brain organotypic cultures are a powerful method for studying cellular and molecular aspects of neuropathologies, as well as the effects of neuroprotective drugs. The main advantages of this method include maintaining the brain structure and its cellular connectivity. However, there is still a lack of studies that better characterize this model, not only to obtain more reliable results, but also to expand its applications. The objective this present project is to expand the characterization of a model of adult human brain using cortical slices obtained from patients undergoing surgery for epilepsy, observing the oxidative metabolism of this tissue by respirometry and citrate synthase activity. We will also assess the maintenance of neuronal function over the days in culture by assessing c-fos protein levels. Furthermore, we will apply our model in studies of infection of the central nervous system by Oropouche virus, determining infected neural phenotypes, apoptotic markers and possible cellular parameters that can be altered as a result this infection. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, GLAUCIA M.; SOUZA, JULIANO P.; MENDES, NIELE D.; PONTELLI, MARJORIE C.; PINHEIRO, NATHALIA R.; NOGUEIRA, GIOVANNA O.; CARDOSO, RICARDO S.; PAIVA, ISADORA M.; FERRARI, GUSTAVO D.; VERAS, FLAVIO P.; et al. Neural Infection by Oropouche Virus in Adult Human Brain Slices Induces an Inflammatory and Toxic Response. FRONTIERS IN NEUROSCIENCE, v. 15, NOV 23 2021. (14/02438-6, 18/06614-4, 14/25681-3)

FERNANDES, ARTUR; MENDES, NIELE DIAS; ALMEIDA, GLAUCIA MARIA; NOGUEIRA, GIOVANNA ORLOVSKI; MACHADO, CARLA DE MORAES; DE CASTRO HORTA-JUNIOR, JOSE DE ANCHIETA; ASSIRATI JUNIOR, JOAO ALBERTO; GARCIA-CAIRASCO, NORBERTO; NEDER, LUCIANO; SEBOLLELA, ADRIANO. Short-Term Free-Floating Slice Cultures from the Adult Human Brain. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 153, NOV 2019. (18/06614-4)

FERNANDES, ARTUR; MENDES, NIELE DIAS; ALMEIDA, GLAUCIA MARIA; NOGUEIRA, GIOVANNA ORLOVSKI; MACHADO, CARLA DE MORAES; DE CASTRO HORTA-JUNIOR, JOSE DE ANCHIETA; ASSIRATI JUNIOR, JOAO ALBERTO; GARCIA-CAIRASCO, NORBERTO; NEDER, LUCIANO; SEBOLLELA, ADRIANO. Short-Term Free-Floating Slice Cultures from the Adult Human Brain. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 153, p. 9-pg., 2019-11-01. (18/06614-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALMEIDA, Glaucia Maria de. Study of central nervous system infection by the Oropouche vírus in adult human brain slice cultures. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.