Stimulation of the hypothalamic-pituitary-adrenal axis by chronic stress and its effect on the progression of the periapical lesion

• Grant number: 18/12438-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2019
• End date: December 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcia Carneiro Valera Garakis
• Grantee: Giovanna Bignoto Minhoto
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

This study will evaluate, in rats, the progression of the periapical lesion to stimulation of the hypothalamic-pituitary-adrenal axis by chronic stress. Will use animal model of periapicopatia and chronic stress, being that the stress will begin 7 days before the induction of the injury. The progression of the lesion will be evaluated in 3 different periods: after 14, 21 and 28 days of the beginning of the lesion induction. The level of blood corticosteroid will be evaluated for confirmation of stress. To evaluate the progression of the periapical lesion will be performed histological and histomorphometric analysis, immunohistochemistry that will evaluate the expression of RANK and RANKL, inflammatory cytokines (IL-1B, IL-6, IL-10 and TNF±) and computerized microtomography of periapical bone destruction. The data will be evaluated by descriptive and inferential statistical tests, according to data obtained, according to their normality, with parametric or non-parametric tests.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MINHOTO, G. B.; KHOURY, R. D.; OROZCO, E. I. F.; PRADO, R. F.; VALERA, M. C.. Effect of chronic unpredictable stress on the progression of experimental apical periodontitis in rats. International Endodontic Journal, v. 54, n. 8, p. 1342-1352, AUG 2021. (18/12438-4, 18/10339-9, 18/01703-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MINHOTO, Giovanna Bignoto. Stimulation of the hypothalamic-pituitary-adrenal axis by chronic stress and its effect on the progression of periapical injury. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.