Nanotechnology-based topical delivery systems for breast cancer chemoprevention

• Grant number: 18/14375-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2019
• End date: May 31, 2023
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Luciana Biagini Lopes
• Grantee: Alexsandra Conceição Apolinário
• Associated research grant: 13/16617-7 - Nanostructured systems for topical delivery and co-localization of chemopreventive and chemoterapeutic agents in the skin and breast tissue, AP.JP 18/13877-1 - Nanocarriers for localized treatment and chemoprevention of breast tumors, AP.JP2
• Associated scholarship(s): 21/06919-2 - Nanoengineered microneedle arrays for enhanced and sustained delivery of the poorly soluble drug fenretinide, BE.EP.PD

Abstract

This project aims at developing innovative strategies for breast cancer chemoprevention based on nanotechnology and modern 3D models. This project is justified by the lack of strategies that are both well accepted and effective for chemoprevention in high-risk population. Herein, we have proposed the development of lipid-based elastic nanovesicles containing ethanol (20-45%) named ethosomes for topical administration and mammary tissue localization of the synthetic retinoid fenretinide. Microscopic techniques, light scattering and nano tracking analysis will be employed for evaluation of morphology, size distribution and zeta potential of ethosomes. To obtain a topical dosage form more easily administered, we propose to incorporate the ethosomes in a gel, and assess its rheological proprieties and stability. The influence of nanocarrier composition on the skin permeability, drug transport in/through the skin and irritation potential will be studied by consolidated methods. Subsequently, optimized formulations will be assessed for their antiproliferative activity in 2D and 3D breast cancer models.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

APOLINARIO, ALEXSANDRA CONCEICAO; HAUSCHKE, LEON; NUNES, JESSICA RIBEIRO; LOPES, LUCIANA BIAGINI. Lipid nanovesicles for biomedical applications: `What is in a name'?. PROGRESS IN LIPID RESEARCH, v. 82, APR 2021. (18/14375-0, 14/50928-2, 18/13877-1)

MOJEIKO, GABRIELA; PASSOS, JULIA SAPIENZA; APOLINARIO, ALEXSANDRA CONCEICAO; LOPES, LUCIANA BIAGINI. opical transdermal chemoprevention of breast cancer: where will nanomedical approaches deliver us. Nanomedicine, v. 16, n. 19, JUL 2021. (18/13877-1, 18/14375-0, 19/00196-9, 20/01208-8, 14/50928-2)

APOLINARIO, ALEXSANDRA CONCEICAO; HAUSCHKE, LEON; NUNES, JESSICA RIBEIRO; LOPES, LUCIANA BIAGINI. Towards nanoformulations for skin delivery of poorly soluble API: What does indeed matter?. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 60, DEC 2020. (18/14375-0, 18/13877-1)

APOLINARIO, ALEXSANDRA CONCEICAO; SALATA, GIOVANNA CASSONE; DE SOUZA, MARCELO MEDINA; CHORILLI, MARLUS; LOPES, LUCIANA BIAGINI. Rethinking Breast Cancer Chemoprevention: Technological Advantages and Enhanced Performance of a Nanoethosomal-Based Hydrogel for Topical Administration of Fenretinide. AAPS PHARMSCITECH, v. 23, n. 4, p. 14-pg., 2022-04-05. (18/13877-1, 14/50928-2, 20/13139-0, 18/14375-0)

ALEXSANDRA CONCEIÇÃO APOLINÁRIO; GIOVANNA CASSONE SALATA; ARTHUR F. R. BIANCO; CLAUDIO FUKUMORI; LUCIANA BIAGINI LOPES. ABRINDO A CAIXA DE PANDORA DOS NANOMEDICAMENTOS: HÁ REALMENTE MUITO MAIS ‘ESPAÇO LÁ EMBAIXO’. Química Nova, v. 43, n. 2, p. 212-225, 2020-05-18. (17/23213-0, 14/50928-2, 18/14375-0, 18/13877-1)

APOLINARIO, ALEXSANDRA CONCEICAO; HAUSCHKE, LEON; NUNES, JESSICA RIBEIRO; LOURENCO, FELIPE REBELLO; LOPES, LUCIANA BIAGINI. Design of multifunctional ethosomes for topical fenretinide delivery and breast cancer chemoprevention. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 623, AUG 20 2021. (14/50928-2, 18/14375-0, 18/13877-1)

MOJEIKO, GABRIELA; APOLINARIO, ALEXSANDRA CONCEICA; SALATA, GIOVANNA CASSONE; CHORILLI, MARLUS; LOPES, LUCIANA B.. Optimization of nanoemulsified systems containing lamellar phases for co-delivery of celecoxib and endoxifen to the skin aiming for breast cancer chemoprevention and treatment. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 646, p. 14-pg., 2022-08-05. (19/26048-6, 18/14375-0, 19/00196-9, 18/13877-1, 14/50928-2)

APOLINARIO, ALEXSANDRA CONCEICAO; HIRATA, AMANDA SOARES; ANJOS MIGUEL, RODRIGO DOS; COSTA-LOTUFO, LETICIA VERAS; PESSOA, ADALBERTO; LA CLAIR, JAMES J.; FENICAL, WILLIAM; LOPES, LUCIANA BIAGINI. Exploring the benefits of nanotechnology for cancer drugs in different stages of the drug development pipeline. Nanomedicine, v. 15, n. 26, p. 4-pg., 2020-09-18. (18/13877-1, 15/17177-6, 18/07661-6, 18/14375-0)