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Endodontic retreatment in single or multiple-visit: sings and symptoms, level of endotoxins and microbial load

Grant number: 18/21882-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2019
Effective date (End): December 31, 2019
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal researcher:Marcia Carneiro Valera Garakis
Grantee:Karollyne Santos Spigariol
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The aim of this study is a) to monitor the microbial load (CFU/mL) and the endotoxin levels (UE/mL) at the beginning, during the retreatment and before the root filling procedure in teeth with secondary endodontic infection performed in single and multiplevisitendodontic retreatment; b) to relate endotoxin levels (UE/mL) and microbial load(CFU/ mL) with the presence of clinical signs and symptoms and the initial volume of the periapical lesion (mm³) by cone-beam computerized tomography (CBCT) analysis; c) to compare the endodontic retreatment in single x multiple-visit as the presence of symptomatology after their conclusion. Regarding this, root canal content collections willbe performed: after the removal of root filling material (S1), after instrumentation withReciproc® system (S2) and after intracanal medication (MIC) (S3) for multiple-visit cases. The content of root canals will be analyzed by antimicrobial activity for microbiological culture (CFU/mL) and the endotoxin levels (EU/mL) by the Limulus Amebocyte Lysate(LAL) test. Also, the volume of the periapical bone destruction will be accessed throughCBCT using Nemotec® software. The data will be analyzed statistically.

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