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Grant number: 15/05397-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2015
Effective date (End): January 31, 2017
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Marcia Carneiro Valera Garakis
Grantee:Cassia Cestari Toia
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated scholarship(s):16/06266-0 - Nanofibers scaffolds with simvastatin: physical, chemical and biological properties, BE.EP.MS


The aims of the present study will be: a) quantify endotoxins and bacterial CFU counts in primary endodontic infections before and after biomechanical preparation (BP) and the use of root canal medication (RCM) in case of multiple-visit treatment; b) to correlate the levels of endotoxins and bacterial CFU counts with the volume of periapical bone destruction (VPBD) determined by Cone Beam Computed Tomography (CBCT) analysis as well as with the development of clinical signs and symptom; c)connect the levels with the volumes of root canals; d)compare the volume of periapical lesions before and after 1 year of endodontic treatment in cases of single-session and multiple session. Thirty teeth with pulp necrosis and apical periodontitis will be selected and submitted to CBCT analysis. After access to pulp cavity, a first root canal sampling will be perform in order to determine the presence of root canal infection. Afterwards, BP will be perform using 2.5% NaOCl followed by the placement of RCM according to the selection group: GI - Calcium Hydroxide [Ca(OH)2] - Ca(OH)2 + Saline Solution (SSL); GII -Calcium Hydroxide paste, (Ultracal®) and GIII - single-visit root canal treatment. A second collect will be made after the BP and then the third after 14-days of RCM. Culture analysis was performed in order to determine antimicrobial activity. The levels of endotoxins were determined by the Limulus assay. The volumes of periapical bone destruction will be accomplished by using CBCT Nemotec® software. In addition, will be sustained a control of endodontic treatment, to compare the volumetric periapical lesions before and after 1 year of treatment protocols. All data will be analyzed statistically

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACHADO, FELIPE PAIVA; KHOURY, RAYANA DUARTE; TOIA, CASSIA CESTARI; FLORES OROZCO, ESTEBAN ISAI; DE OLIVEIRA, FELIPE EDUARDO; DE OLIVEIRA, LUCIANE DIAS; DA ROSA CARDOSO, FLAVIA GOULART; VALERA, MARCIA CARNEIRO. Primary versus post-treatment apical periodontitis: microbial composition, lipopolysaccharides and lipoteichoic acid levels, signs and symptoms. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 9, p. 3169-3179, . (18/01703-9, 15/05397-1)
CAVALLI, DAIANA; TOIA, CASSIA CESTARI; FLORES OROZCO, ESTEBAN ISAI; KHOURY, RAYANA DUARTE; DA ROSA CARDOSO, FLAVIA GOULART; ALVES, MARCELO CORREA; TALGE CARVALHO, CLAUDIO ANTONIO; VALERA, MARCIA CARNEIRO. Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study. JOURNAL OF ENDODONTICS, v. 43, n. 8, p. 1237-1245, . (15/05397-1, 14/25789-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TOIA, Cassia Cestari. Single visit x multiple visit endodontic treatment: correlation of success after 1 year of treatment with endotoxin levels, microbial load and signs / symptoms. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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