Single visit x multiple visit endodontic treatment: correlation of success after 1 year of treatment with endotoxin levels, microbial load and signs / symptoms

Author(s)

Cassia Cestari Toia

• Document type: Master's Dissertation
• Press: São José dos Campos. 2017-05-12.
• Institution: Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos
• Defense date: 2017-01-26
• Advisor: Marcia Carneiro Valera; Flávia Goulart da Rosa Cardoso

Abstract

The aim of this study was: a) to investigate microbial load (UFC/mL) and endotoxin levels (EU/mL) in primary endodontic infections in permanent teeth submitted to endodontic treatment in single visit (SV) versus multiple visit (MV) treatment; b) to correlate EU/mL and UFC/mL with clinical signs and symptoms and root canal and periapical lesion volume (mm³) using cone bean computed tomography (CBCT); c) to compare the periapical lesion volume (mm³) before and after one year follow up in the SV and MV groups; d) to compare the reduction of periapical lesion volume (mm³) after one year of treatment with EU/mL and CFU/mL in the beginning of the treatment and before the obturation process; E) to compare the success rate after one year follow up between SV and MV.Twenty teeth with pulp necrosis and periapical lesion were selected and submitted to CBCT. Root canal contents were collected: after coronary opening (S1), after the instrumentation with Reciproc® and irrigation with sodium hipoclorite 2,5% (S2), after irrigation with EDTA 17% (S3) and after intracanal medication for 14 days with calcium hydroxide paste and physiological saline solution (S4). Antimicrobial activity was evaluated by microbiological culture and endotoxin levels by the Lysed Amebocyte Limulus test. Root canal volumetry and bone destruction were analyzed through the CBCT using Nemotec® software. Finally, one year follow up visit was conducted to compare both treatments regarding the periapical lesion healing process correlating the volume of lesions with clinical, radiographic and initial tomography findings. Data obtained were statistically analyzed. UFC/mL and EU/mL levels decreased after biomechanical preparation, but intracanal medication and EDTA 17% did not interfere the results; CFU/mL and EU/mL were not related to signs/symptoms and periapical lesion volume. However there was a correlation between the periapical lesion volume and the signs/symptoms. There was no statistical difference between the SV and SM treatments about the reduction of the periapical lesions after one year follow up and there was no relation between CFU/mL and EU/mL with regression of the periapical lesion. It was concluded, therefore, that the type of intervention (SV x MV) did not influence the success of endodontic treatment. (AU)

• FAPESP's process: 15/05397-1 - ENDOTOXINS QUANTIFICATION AND ITS RELATIONSHIP WITH SIGNS AND SYMPTOMS: SINGLE-VISIT VERSUS MULTIPLE-VISIT ENDODONTIC TREATMENT- CONTROL AFTER 1 YEAR CBCT
• Grantee: Cassia Cestari Toia
• Support Opportunities: Scholarships in Brazil - Master