Study of the role of miR-203-3p in adipose tissue metabolism and in the pathogenesis of metabolic diseases in mice

• Grant number: 18/21635-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): March 01, 2019
• Effective date (End): February 28, 2023
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Marcelo Alves da Silva Mori
• Grantee: Gabriel Palermo Ruiz
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics, AP.TEM

Abstract

The Metabolic Syndrome affects 25% of the adult population, emerging as one of the largest public health concerns worldwide, being Obesity and insulin resistance extremely relevant in this context. Caloric restriction and physical exercise appear as important interventions to treat metabolic diseases, however, the cellular and molecular components that participate in the beneficial effects of these interventions are not completely elucidated. It is known that adipose tissue microRNAs (miRNAs) are regulated under these conditions and that miR-203-3p is articularly decreased in Obesity and increased in response to physical exercise and caloric restriction. Preliminary data from our group point to miR203-3p as a candidate to mediate metabolic adaptation under energetic stress conditions. However, this miRNA is barely explored in the context of metabolism and studies are required to characterize its functions in vivo. Thus, we propose to generate adipocyte-specific mir203 knockout mice seeking to understand the function of miR-203-3p in adipose tissue in vivo, its role in the metabolic adaptation to caloric restriction and physical exercise, as well as its possible therapeutic role in metabolic disorders. In parallel, we will evaluate, in vivo and in vitro, a possible role for adipose tissue miR-203-3p in intertissue communication. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRANDAO, BRUNA B.; MADSEN, SOREN; RABIEE, ATEFEH; OLIVERIO, MATTEO; RUIZ, GABRIEL P.; FERRUCCI, DANILO L.; BRANQUINHO, JESSICA L.; RAZOLLI, DANIELA; PINTO, SILAS; NIELSEN, THOMAS S.; et al. Dynamic changes in DICER levels in adipose tissue control metabolic adaptations to exercise. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 117, n. 38, p. 23932-23941, SEP 22 2020. (17/03423-0, 15/01316-7, 17/04377-2, 12/06238-6, 15/03292-8, 17/01184-9, 12/04079-8, 18/21635-8, 17/07975-8)

RUIZ, GABRIEL PALERMO; CAMARA, HENRIQUE; FAZOLINI, NARAYANA P. B.; MORI, MARCELO A.. Extracellular miRNAs in redox signaling: Health, disease and potential therapies. Free Radical Biology and Medicine, v. 173, p. 170-187, SEP 2021. (17/01339-2, 17/01184-9, 18/21635-8, 17/23920-9, 17/07975-8)