Genome comparative analysis of four Xanthomonas albilineans isolates and prospection of target genes for association studies with known phenotypes

• Grant number: 18/24646-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2019
• End date: July 31, 2021
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Marie-Anne Van Sluys
• Grantee: Raquel Paulini Miranda
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case, AP.BIOEN.TEM

Abstract

Xanthomonas albilineans is the agent responsible for the leaf scald in sugarcane. This culture represents one of the relevant cultures of the Brazilian agribusiness for being an important worldwide commodity. Four field isolates were sequenced in a partnership established in 2015 between Dr. Silvana Creste (IAC) and Dr. Marie-Anne Van Sluys. Preliminary analysis of these bacterial isolates revealed the presence of genomic segments unique to each isolate, as well as highly conserved regions. Based on the collected data and bioinformatics analysis - genomic clustering, maximum entropy analysis, gene repertoire analyzes and others - we propose to carry out a comparative study among the different isolates. The proposed study aims to characterize and prospect sequences for further association studies between genotype and known phenotypes - variable disease severity levels within a range of symptoms. This research project is linked to the FAPESP thematic project "GEMAC - Genes genomes and transposable elements contribution to plant-microbe interaction: a sugarcane study case", process 2016/17545-8. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA, RAQUEL P.; TURRINI, PAULA C. G.; BONADIO, DORA T.; ZERILLO, MARCELO M.; BERSELLI, ARTHUR P.; CRESTE, SILVANA; VAN SLUYS, MARIE-ANNE. Genome Organization of Four Brazilian Xanthomonas albilineans Strains Does Not Correlate with Aggressiveness. MICROBIOLOGY SPECTRUM, v. 11, n. 3, p. 17-pg., 2023-04-13. (08/52074-0, 18/23646-7, 19/05424-0, 18/24646-0, 16/17545-8)