Proteomic profile of neural stem cells and neurospheres infected with Zika virus and Dengue virus

• Grant number: 18/25439-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2019
• End date: March 31, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Daniel Martins-de-Souza
• Grantee: Danielle Gouvêa Junqueira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In 2015, the Brazilian Zika virus outbreak was accompanied by an increase in the number of cases of microcephaly, which involves severe changes in brain development, indicating a possible association of viral infection with these brain malformations. The molecular mechanisms that promote these changes are not yet fully understood. Therefore, the investigation of the processes involving the development of the disease and the biochemical pathways that it affects is fundamental. For this purpose, study models using human-induced pluripotency stem cells (hiPSC), differentiated into neural cells, can be studied as a neurodevelopment model. Here, human neural progenitors (hNPC) have been infected with strains of zika and dengue virus. The infection was carried out in the neural progenitor state and also during its differentiation into neurospheres, to analyze in vitro the changes caused by viral infection in these models, which are the type of cell most affected by the Zika virus. The samples, infected or not with a strain of the virus, were subjected to quantitative proteomic analysis on a large scale, in order to analyze differences and similarities in relation to the effects of the Brazilian and African viral strains, as well as to compare the Zika virus infection with the dengue virus. The analysis of the cellular proteome allowed the identification of possible metabolic pathways and proteins that may be related to the mechanisms and development of the disease. And we were also able to point out potential differences between the cellular response to the different strains of virus analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, JULIANA MINARDI; GOUVEA-JUNQUEIRA, DANIELLE; ZUCCOLI, GIULIANA S.; GOUVEIA PEDROSA, CAROLINA DA SILVA; BRANDAO-TELES, CAROLINE; CRUNFLI, FERNANDA; ANTUNES, ANDRE S. L. M.; CASSOLI, JULIANA S.; KARMIRIAN, KARINA; SALERNO, JOSE ALEXANDRE; et al. Zika Virus Strains and Dengue Virus Induce Distinct Proteomic Changes in Neural Stem Cells and Neurospheres. Molecular Neurobiology, v. N/A, p. 15-pg., 2022-06-22. (18/25439-9, 14/21035-0, 17/25055-3, 18/14666-4, 19/00098-7, 20/10282-7, 17/25588-1)

GOUVEA-JUNQUEIRA, DANIELLE; FALVELLA, ANA CAROLINE BRAMBILLA; ANTUNES, ANDRE SARAIVA LEAO MARCELO; SEABRA, GABRIELA; BRANDAO-TELES, CAROLINE; MARTINS-DE-SOUZA, DANIEL; CRUNFLI, FERNANDA. Novel Treatment Strategies Targeting Myelin and Oligodendrocyte Dysfunction in Schizophrenia. FRONTIERS IN PSYCHIATRY, v. 11, APR 30 2020. (17/25588-1, 17/25055-3, 18/03673-0, 19/22398-2, 18/10362-0, 18/25439-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

JUNQUEIRA, Danielle Gouvêa. Proteomic profile of neural stem cells and neurospheres infected with dengue and zikva virus strains. 2021. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.