No clinical toxicology studies of red propolis and its botanical source Dalbergia ecastophyllum (L.) Taub.

• Grant number: 17/26252-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: July 31, 2022
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Jairo Kenupp Bastos
• Grantee: Jennyfer Andrea Aldana Mejía
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM

Abstract

Propolis is a natural resinous product, comprising a mixture of compounds collected by bees from plants and mixed with wax of their glands. Propolis performs important biological functions within the hive, for which its protection effect against pathogens and parasites stands out. Propolis has broad traditional use, and many biological activities makes Brazilian propolis highly valued in Asian countries. One of the most prominent types of propolis is the red one, which botanical source is Dalbergia ecastophyllum (L) Taub. (Fabaceae). Chemically it is characterized by the presence of flavonones, flavonols, xanthones, triterpenes, catechins, chalcones, aurones and guttiferones. Extracts and some isolated compounds are considered free radical scavengers, antimicrobials, toxic against tumor cell lines and anti-inflammatory, which makes red propolis a product with high potential for the development of pharmaceutical products. However, there are few studies for guaranteeing the chemical characterization and safety for the use of red propolis. The main goal of this proposal is to carry out in vivo and in vitro evaluations of the possible toxic effects of both the crude extract of the red propolis and its vegetal source. In order to achieve this aim, the in vitro cytotoxic activities of the crude extracts of D. ecastophyllum and of propolis will be evaluated by the XTT assay using normal V79 cells; the in vitro genotoxic activity of the extracts will be evaluated by performing the micronucleus assay and in vivo assay using mouse erythrocytes. Acute toxicity tests for single dose (14 days) and subchronic toxicity in repeated doses for 90 days of the oral red propolis extract will be carried out, by monitoring the behavioral changes, and at 90th day hematological, biochemical, macro and microscopical morphological analyses will be performed. Considering the need of chemical characterization of the extract, we are also proposing to carry out the isolation, by chromatographic means, of the major compounds of the red propolis, as a contribution of standards for the development of analytical methods by other members of the research team.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIGLIORE, LETIZIA J.; CCANA-CCAPATINTA, V, GARI; CURLETTI, GIANFRANCO; CASARI, SONIA A.; BIFFI, GABRIEL; MEJIA, JENNYFER A. A.; CARVALHO, JEAN C. A. S.; BASTOS, JAIRO K.. new species of jewel beetle (Coleoptera, Buprestidae, Agrilus) triggers the production of the Brazilian red propoli. NATURWISSENSCHAFTEN, v. 109, n. 2, APR 2022. (17/04138-8, 18/13700-4, 17/26252-7)

CCANA-CCAPATINTA, GARI VIDAL; ALDANA MEJIA, JENNYFER ANDREA; TANIMOTO, MATHEUS HIKARU; GROPPO, MILTON; ANDRADE SARMENTO DE CARVALHO, JEAN CARLOS; BASTOS, JAIRO KENUPP. Dalbergia ecastaphyllum (L.) Taub. and Symphonia globulifera L.f.: The Botanical Sources of Isoflavonoids and Benzophenones in Brazilian Red Propolis. Molecules, v. 25, n. 9, MAY 2020. (17/04138-8, 17/26252-7, 18/13700-4, 19/01697-1)

ALDANA-MEJIA, JENNYFER ANDREA; DE MIRANDA, ALINE MAYRINK; CCANA-CCAPATINTA, GARI V.; DE ARAUJO, LUCIANA SILVA; TAVARES, DENISE C.; RAMALHO, FERNANDO S.; BASTOS, JAIRO K.. Long-term Effects of Brazilian Red Propolis Ethanolic Extract on Biochemistry and Histopathological Parameters of Wistar Rats. FASEB JOURNAL, v. 36, p. 1-pg., 2022-01-01. (17/26252-7, 17/04138-8)

ALDANA-MEJIA, JENNYFER A.; CCANA-CCAPATINTA, V, GARI; SQUARISI, IARA S.; NASCIMENTO, SAMUEL; TANIMOTO, MATHEUS H.; RIBEIRO, VICTOR P.; ARRUDA, CAROLINE; NICOLELLA, HELOIZA; ESPERANDIM, TABATA; RIBEIRO, ARTHUR B.; et al. onclinical Toxicological Studies of Brazilian Red Propolis and Its Primary Botanical Source Dalbergia ecastaphyllu. Chemical Research in Toxicology, v. 34, n. 4, p. 1024-1033, APR 19 2021. (17/26252-7, 17/04138-8)

ALDANA-MEJIA, JENNYFER A.; CCANA-CCAPATINTA, V, GARI; RIBEIRO, VICTOR PENA; ARRUDA, CAROLINE; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO RICARDO; BASTOS, JAIRO KENUPP. validated HPLC-UV method for the analysis of phenolic compounds in Brazilian red propolis and Dalbergia ecastaphyllu. Journal of Pharmaceutical and Biomedical Analysis, v. 198, MAY 10 2021. (17/26252-7)

ALDANA-MEJIA, JENNYFER ANDREA; DE MIRANDA, ALINE MAYRINK; CCANA-CCAPATINTA, GARI VIDAL; DE ARAUJO, LUCIANA SILVA; RIBEIRO, VICTOR PENA; ARRUDA, CAROLINE; NASCIMENTO, SAMUEL; SQUARISI, IARA; ESPERANDIM, TABATA; DE FREITAS, KAROLINE S.; et al. Genotoxicity and toxicological evaluations of Brazilian red propolis oral ingestion in a preclinical rodent model. Journal of Ethnopharmacology, v. 303, p. 12-pg., 2023-03-01. (17/04138-8, 17/26252-7)

ALDANA-MEJIA, JENNYFER A.; CCANA-CCAPATINTA, GARI, V; RIBEIRO, VICTOR PENA; ARRUDA, CAROLINE; VENEZIANI, RODRIGO C. S.; AMBROSIO, SERGIO RICARDO; BASTOS, JAIRO KENUPP. A validated HPLC-UV method for the analysis of phenolic compounds in Brazilian red propolis and Dalbergia ecastaphyllum. Journal of Pharmaceutical and Biomedical Analysis, v. 198, p. 7-pg., 2021-03-20. (17/26252-7)

ALDANA-MEJIA, JENNYFER A.; CCANA-CCAPATINTA, GARI, V; SQUARISI, IARA S.; NASCIMENTO, SAMUEL; TANIMOTO, MATHEUS H.; RIBEIRO, VICTOR P.; ARRUDA, CAROLINE; NICOLELLA, HELOIZA; ESPERANDIM, TABATA; RIBEIRO, ARTHUR B.; et al. Nonclinical Toxicological Studies of Brazilian Red Propolis and Its Primary Botanical Source Dalbergia ecastaphyllum. Chemical Research in Toxicology, v. 34, n. 4, p. 10-pg., 2021-03-15. (17/04138-8, 17/26252-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MEJÍA, Jennyfer Andrea Aldana. Chemical characterization and toxicological studies of Brazilian red propolis and its botanical source Dalbergia ecastaphyllum (L.) Taub. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.