In vitro evaluation of the metabolism of the major compounds of Araucaria sp brown propolis

• Grant number: 22/00659-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2022
• End date: February 28, 2023
• Field of knowledge: Health Sciences - Pharmacy - Pharmacognosy
• Principal Investigator: Sergio Ricardo Ambrosio
• Grantee: Victor Pena Ribeiro
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM

Abstract

The Brazilian propolis produced by Apis mellifera called green, brown and red have great economic importance, due to their biological activities and export to several countries, such as Japan and China,and productionof approximately 200 tons per year. The main botanical origins of Brazilian propolis are: Baccharis dracunculifolia for green propolis; Araucaria sp, Eucalyptus sp and others for brown propolis; and Dalbergia ecastophyllum for the red propolis. In 2005, ANVISA published a technical note on the registration of products containing propolis, as well as the RDC no. 24/2011 authorizing the registration of propolis-based medicines. However, to date nonemedicine has been registered with ANVISA. There are many gaps to be filled, since ANVISA demands the execution of chemical, analytical, pharmacological and technological studies to guarantee safety, efficacy and quality in the development of new medicines. Thus, it is proposed to carry out complementary steps in phytochemical studies to obtain standardized extracts and compounds to be used in the development of analytical methods; in obtaining semi-synthetic derivatives; in stability and degradation studies; in bioaccessibility, biotransformation, pharmacokinetic and pharmacodynamic studies; in in silico studies to determine chemical structure-activity relationship and docking analyses; in the development of pharmaceutical formulations; in both in vitro and invivo toxicological studies; in studies of genotoxicity and mutagenicity; in anti-inflammatory, immunomodulatory, antimicrobial, antiparasitic, antiproliferative, healing and gastroprotective assays, among others. The ultimate goal of this proposal is to add value to the Brazilian beekeeping sector.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, VICTOR PENA; ALDANA-MEJIA, JENNYFER ANDREA; ARRUDA, CAROLINE; CANDIDO, ANA CAROLINA BOLELA BOVO; MAGALHAES, LIZANDRA GUIDI; OLIVEIRA, NICOLI DIAS; VENEZIANI, RODRIGO CASSIO SOLA; BASTOS, JAIRO KENUPP; AMBROSIO, SERGIO RICARDO. A RP-HPLC-DAD method for analysis of Brazilian southeast brown propolis and its leishmanicidal properties. BIOMEDICAL CHROMATOGRAPHY, v. 37, n. 8, p. 11-pg., 2023-04-18. (22/00659-1, 17/04138-8)

RIBEIRO, VICTOR PENA; ALDANA MEJIA, JENNYFER ANDREA; RODRIGUES, DEBORA MUNHOZ; ALVES, GABRIEL ROCHA; PINHEIRO, ANA MARIA DE FREITAS; TANIMOTO, MATHEUS HIKARU; BASTOS, JAIRO KENUPP; AMBROSIO, SERGIO RICARDO. Brazilian Brown Propolis: an Overview About Its Chemical Composition, Botanical Sources, Quality Control, and Pharmacological Properties. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 33, n. 2, p. 12-pg., 2023-02-24. (22/00659-1, 17/04138-8)