In vitro, in vivo and in silico studies of the antineoplastic activity of guttiferone e in melanoma

• Grant number: 18/25770-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2019
• End date: February 28, 2021
• Field of knowledge: Biological Sciences - Genetics - Mutagenesis
• Principal Investigator: Denise Crispim Tavares
• Grantee: Arthur Barcelos Ribeiro
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
• Associated research grant: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM

Abstract

Melanoma (MLA) has a heterogeneous and complex etiology resulting from the interaction of genetic, immunological and environmental factors, which play essential roles in all phases of its development. The best therapeutic responses for the treatment of MLA are based on the infusion of cytotoxic drugs, limited by immunosuppression, resistance and high toxicity. Several molecular changes involved in the initiation and progression of MLA have been elucidated. However, advances in therapy are still limited. In the search for new molecules for treatment, as well as in reducing side effects of existing treatments, products of natural origin are highlighted in cancer chemotherapy, and can be used in treatments in isolation or combined with others antineoplastic drugs. In view of the aspects mentioned, Guttiferone E (GE), one of the major constituents of Brazilian red propolis, has antiproliferative activity based on the literature.However, studies that report the effect of GE on the carcinogenesis of MLA are non-existent and molecular mechanisms still need to be better explored. Thus, it is relevant to understand the biological functions of this molecule, in order to obtain knowledge that contributes to the development of more effective therapeutic approaches and the improvement of therapies already established for the MLA. Thus, the present study aims to evaluate the antitumor potential of GE in MLA. To this end, functional assays of cellular morphology and viability, clonogenicity, cell cycle, apoptosis, cellular migration and adhesion will be conducted after treatment with GE in the human melanoma (A-375) and murine (B16-F10) strains, as well as on the non-tumor human keratinocyte (HaCat) strain. This research may provide perspectives in the identification of new therapeutic strategies for the treatment of MLA. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIBEIRO, ARTHUR BARCELOS; NICOLELLA, HELOIZA DINIZ; DOMINGOS DA SILVA, LUCAS HENRIQUE; ALDANA MEJIA, JENNYFER ANDREA; TANIMOTO, MATHEUS HIKARU; AMBROSIO, SERGIO RICARDO; BASTOS, JAIRO KENUPP; ORENHA, RENATO PEREIRA; TAME PARREIRA, RENATO LUIS; TAVARES, DENISE CRISPIM. Guttiferone E Displays Antineoplastic Activity Against Melanoma Cells. Planta Medica, v. N/A, p. 10-pg., 2022-09-28. (18/25770-7, 17/04138-8)