Effects of lipid-core nanocapsules with acetyleugenol in melanomas: in vivo and in vitro studies

• Grant number: 10/19802-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2012
• Effective date (End): March 31, 2015
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Sandra Helena Poliselli Farsky
• Grantee: Carine Cristiane Drewes
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Nanotechonlogy has attracted intense interest in several areas, including pharmaceuticals. Among the various nanotechnology products available, the pharmaceutical nanoparticle systems have been highlighted by driving drugs into sites of action and acting as drug carriers, increasing the therapeutic efficacy. These characteristics are fundamental to the treatment of many diseases, including cancer. Despite the numerous advantages of anticancer nanocapsules, their pharmacological and toxicological parameters have not been fully comprehended. In this context, this study aims to evaluate the anti-carcinogenic activity and possible toxic effects of eugenol nanocapsules and of eugenol solution. Male C57BL6 mice will be i.v. treated with fluorophore (Rhodamine B)-labeled eugenol nanocapsules or with eugenol solution. The nanocapsules distribution in the microcirculation in the liver or in the B16F10-induced melanoma tumor mass in the subcutaneous dorsal tissue; leukocyte-endothelial interactions; formation of thrombus and hemorrhagic areas, and therapeutic efficacy by measuring the kinetics of tumor formation in the dorsal subcutaneous tissue will be measured by intravital microscopy. Additionally, SK-Mel-28 human melanoma and HUVEC human endothelium cell lines will in vitro treat with the compounds to assess cell viability, cell proliferation, cell cycle and DNA fragmentation by flow cytometry assay; cell adhesion and production of nitric oxide will be quantified by spectrophotometry assay; cell migration in Matrigel® and tissue morphology in hematoxylin and eosin stained tissues will be monitored by optical microscopy. Data obtained in this study may indicate in vitro and in vivo studies to evaluate the therapeutic and toxic action of anti-cancer nanocapsules, and they may contribute to the comprehension of toxic actions of eugenol in the microcirculatory network. Additionally, this project will contribute to the establishment of protocols for study nanotechnology by intravital microscopy, which has not been employed in Brazil, and consolidate the collaboration with Drs Silvia Guterres and Adriana Polhmann´s groups, from the Federal University of Rio Grande do Sul, and specialized in the production of nanocompounds for pharmaceutical application.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VASCONCELOS, STANLEY N. S.; DREWES, CARINE C.; KANDA KUPA, LEONARD DE VINCI; FARSKY, SANDRA H. P.; STEFANI, HELIO A.. Evaluation of toxicity on epithelial and tumor cells of biaryl dipeptide tyrosines. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, v. 114, p. 1-7, MAY 23 2016. (13/17960-7, 12/00424-2, 10/19802-1)

DREWES, C. C.; DIAS, R. Y.; BRANCO, V. G.; CAVALCANTE, M. F.; SOUZA, J. G.; ABDALLA, D. S. P.; CHUDZINSKI-TAVASSI, A. M.; FARSKY, S. H. P.. Post-transcriptional control of Amblyomin-X on secretion of vascular endothelial growth factor and expression of adhesion molecules in endothelial cells. Toxicon, v. 101, p. 1-10, JUL 2015. (09/08584-6, 13/07467-1, 12/06944-8, 10/19802-1)

DREWES, CARINE C.; ALVES, ALINE DE C. S.; HEBEDA, CRISTINA B.; COPETTI, ISABELA; SANDRI, SILVANA; UCHIYAMA, MAYARA K.; ARAKI, KOITI; GUTERRES, SILVIA S.; POHLMANN, ADRIANA R.; FARSKY, SANDRA H.. Role of poly(epsilon-caprolactone) lipid-core nanocapsules on melanoma-neutrophil crosstalk. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 12, p. 7153-7163, 2017. (14/07328-4, 10/19802-1)

MACHADO, ISABEL DAUFENBACK; SANTIN, JOSE ROBERTO; DREWES, CARINE CRISTIANE; GIL, CRISTIANE DAMAS; OLIANI, SONIA MARIA; PERRETTI, MAURO; POLISELLI FARSKY, SANDRA HELENA. Alterations in the profile of blood neutrophil membrane receptors caused by in vivo adrenocorticotrophic hormone actions. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v. 307, n. 9, p. E754-E763, NOV 1 2014. (10/16828-0, 10/19802-1, 10/17175-0, 10/08402-2)

LACERDA, JESSICA ZANI; DREWES, CARINE CRISTIANE; OLIVEIRA MIMURA, KALLYNE KIOKO; ZANON, CAROLINE DE FREITAS; ANSARI, TAHERA; GIL, CRISTIANE DAMAS; GRECO, KARIN VICENTE; POLISELLI FARSKY, SANDRA HELENA; OLIANI, SONIA MARIA. Annexin A1(2-26) Treatment Improves Skin Heterologous Transplantation by Modulating Inflammation and Angiogenesis Processes. FRONTIERS IN PHARMACOLOGY, v. 9, SEP 10 2018. (13/07487-2, 12/13041-4, 12/21603-2, 16/02012-4, 10/19802-1, 14/07328-4)

ALI, BAKAT; KUPA, LEONARD D. KANDA; HELUANY, CINTIA S.; DREWES, CARINE C.; VASCONCELOS, STANLEY N. S.; FARSKY, SANDRA H. P.; STEFANI, HELIO A.. Cytotoxic effects of a novel maleimide derivative on epithelial and tumor cells. BIOORGANIC CHEMISTRY, v. 72, p. 199-207, JUN 2017. (13/17960-7, 12/17954-4, 12/00424-2, 10/19802-1, 14/07328-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DREWES, Carine Cristiane. Effects of lipid-core nanocapsules with acetyleugenol in melanomas: in vivo and in vitro studies. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.