Study of self-assembled monolayers of peptides and their application in immunosensors for detecting Pancreatic Cancer biomarkers

• Grant number: 18/18231-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2019
• End date: October 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Júlia Pinto Piccoli
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

Early detection of diseases such as Pancreatic Cancer requires new devices that need to be inexpensive, easy to handle, with rapid, sensitive and selective responses, and that can be used outside specialized laboratories (i.e. point-of-care). This is possible with electrochemical immunosensors made of nanostructured films, in which effective immobilization of biomolecules is obtained to recognize the biomarker of clinical relevance. Nanostructured peptide films containing an active redox molecule are useful for immunosensors because they are stable and have controllable physicochemical properties. This project is aimed at the synthesis of peptides and fabrication of Self-Assembled Monolayers (SAMs) containing redox molecules such as TOAC (2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) and methylene blue. A layer of antibodies of CA19-9, a Pancreatic Cancer biomarker, will be deposited on these peptide films, thus forming an electrochemical immunosensor for the detection and quantification of CA19-9. Techniques such as Sum-Frequency Generation spectroscopy (SFG) and Polarization-Modulated Infrared Reflection Absorption Spectroscopy (PM-IRRAS) will be used to characterize the films, while detection will be made with electrochemical impedance and/or impedance spectroscopy. It is expected that the performance of the immunosensors will be optimized in terms of selectivity and sensitivity, so that they can be used in any immunosensor of clinical relevance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PICCOLI, JULIA P.; SOARES, ANDREY C.; OLIVEIRA, JR., OSVALDO N.; CILLI, EDUARDO M.. Nanostructured functional peptide films and their application in C-reactive protein immunosensors. BIOELECTROCHEMISTRY, v. 138, APR 2021. (18/22214-6, 13/07600-3, 18/18953-8, 17/24839-0, 18/18231-2)