Multicentric clinicopathological and proteomic study of adenomatoid odontogenic tumors

• Grant number: 19/02539-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2019
• End date: July 31, 2020
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Pablo Agustin Vargas
• Grantee: Ana Luiza Oliveira Corrêa Roza
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Odontogenic tumors are uncommon in the practice of oral pathology and have clinical behavior that varies according to their clinical, radiographic and microscopic characteristics. Adenomatoid odontogenic tumors (AOT) are expansive, slow-growing and progressive tumors, located commonly in the anterior region of the maxilla of children. Ameloblastomas are odontogenic tumors with potentially infiltrative and aggressive biological behavior, with considerable rates of recurrence, located mainly in the posterior region of the mandible of young adults, demonstrating mutations in BRAFV600E and SMO genes. Ameloblastic carcinoma (AC) represents the malignant counterpart of ameloblastoma and a third of patients develop long-term lung metastases. Proteomics analysis has been used to reveal the large-scale protein expression of various neoplasms, however, this technique has not yet been applied in the context of odontogenic tumors. The aim of this study is to evaluate the clinicopathological and proteomic profile of AOT, ameloblastoma, and AC of three countries, identifying prognostic markers for aggressive lesions. Twenty samples with representative material will be collected from paraffin blocks acquired from the archives of four Oral Pathology laboratories in Brazil, Guatemala, and South Africa, with 10 cases of AOT, 5 cases of ameloblastoma and 5 cases of AC that will be submitted to mass spectrometry linked to liquid chromatography for the proteomic study. Protein identification will be based on the UniProt library. Bioinformatics analysis to determine the sub- or super-expression of each component will be carried out using different software, such as the MaxQuant package and SIMCA 14.1. The prognostic potential of the proteins that obtained a statistically significant result will be validated by means of immunohistochemical reactions in a convenience sample composed of twice the number of cases of each tumor, through correlation with clinicopathological data and survival. The use of these techniques may contribute to the treatment of affected patients. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA CORREA ROZA, ANA LUIZA; CARLOS, ROMAN; VAN HEERDEN, WILLIE F. P.; BENEVENUTO DE ANDRADE, BRUNO AUGUSTO; MOSQUEDA-TAYLOR, ADALBERTO; SANTOS-SILVA, ALAN ROGER; LOPES, MARCIO AJUDARTE; DE ALMEIDA, OSLEI PAES; ROMANACH, MARIO JOSE; VARGAS, PABLO AGUSTIN. n international collaborative study of 105 new cases of adenomatoid odontogenic tumor. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 132, n. 3, p. 327-338, SEP 2021. (19/02539-0)

OLIVEIRA CORREA ROZA, ANA LUIZA; KOWALSKI, LUIZ PAULO; WILLIAM JR, WILLIAM NASSIB; DE CASTRO JR, GILBERTO; FREITAS CHAVES, ALINE LAUDA; DAMACENO ARAUJO, ANNA LUIZA; PRADO RIBEIRO, ANA CAROLINA; BRANDAO, THAIS BIANCA; LOPES, MARCIO AJUDARTE; VARGAS, PABLO AGUSTIN; et al. Oral leukoplakia and erythroplakia in young patients: a systematic review. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 131, n. 1, p. 73-84, JAN 2021. (18/02233-6, 19/02539-0)

ROZA, ANA LUIZA OLIVEIRA CORREA; SOUSA, EMANUEL MENDES; LEITE, AMANDA ALMEIDA; AMARAL-SILVA, GLEYSON KLEBER; MORAIS, THAYNA MELO DE LIMA; WAGNER, VIVIAN PETERSEN; SCHUCH, LAUREN FRENZEL; VASCONCELOS, ANA CAROLINA UCHOA; DE ARRUDA, JOSE ALCIDES ALMEIDA; MESQUITA, RICARDO ALVES; et al. Central odontogenic fibroma: an international multicentric study of 62 cases. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 131, n. 5, p. 549-557, MAY 2021. (19/02539-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ROZA, Ana Luiza Oliveira Corrêa. Multicentric clinicopathologic study of adenomatoid odontogenic tumors. 0000. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.