Soybean (Glycine max (L.) Merrill) is one of the world's major oil crops. This culture is too sensitive to environmental factors, and water deficiency is a particularly limiting factor for its development and production. However, the bioregulator melatonin (N-acetyl-5-methoxytryptamine) can alleviate the harmful effects caused by stress and increase its productive potential. MEL acts in the process of reproduction, germination and growth, and the promotion of primary and secondary rooting structures. The objective of the present research is to analyze the effects of the exogenous application of melatonin on soybean seeds, evaluating germination and physiological parameters of plants under water deficit. Therefore, an exogenous application of the MEL in seeds will be performed if soybean cv. Intact under osmotic stress, and the second round of plants starting the reproductive phase. The test will be realized: germination and dry mass evaluation, to select the better doses. Subsequently, will be applied melatonin to the seeds, conditioning the irrigation suspension regime and rehydration. The physiological performance of the plants will be analyzed from the recovery hydric conditions of the plants. Analysis of gas exchange, chlorophyll fluorescence and biochemical analysis of antioxidant enzymes (SOD, CATand APX). A completely randomized 5 x 2 factorial design (5 modes of applications x 2 water regimes) with six replicates per treatment will be used. The results will be analyzed by analysis of variance (ANOVA, p <0.05). The mean values will be compared by the Tukey test (p <0.05).
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