Oxidative stress in feline obesity

• Grant number: 18/19683-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2019
• End date: December 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Breno Fernando Martins de Almeida
• Grantee: Tainara de Oliveira Martins
• Host Institution: Centro Universitário Faculdades Integradas de Ourinhos. Fundação Educacional Miguel Mofarrej. Ourinhos , SP, Brazil

Abstract

Obesity is considered an epidemic condition of extreme importance in feline practice, as it can affect up to 35% of the population. In addition to decreasing life expectancy, obesity is associated with cardiovascular disease, diabetes, hyperthyroidism, liver disorders and some cancers. Oxidative stress is widely related to obesity, as it promotes the release of inflammatory cytokines that lead to the production of reactive oxygen species, generating cellular damage that may be irreversible. The present study will evaluate the oxidative stress associated with obesity in the feline species, testing the hypothesis that total antioxidant capacity, total oxidant capacity and lipid peroxidation are dependent on the degree of obesity. Thirty felines will be selected to the study, divided equally between the control, overweight and obese groups, after clinical-laboratory evaluation. The biochemical analyzes will be carried out in spectrophotometer and the total antioxidant capacity will be determined by three methods (antioxidant capacity equivalent to trolox, plasma ferric reduction capacity and antioxidant reducing cupric capacity), total oxidant capacity, lipid peroxidation by thiobarbituric acid reactive substances, besides the antioxidants uric acid and albumin determined by commercial reagents. The variables will be tested for normality and homoscedasticity and the differences between the groups will be verified by ANOVA tests with Dunn or Kruskall-Walis post-test with Tukey post-test, being considered significant when p <0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, TAINARA DE OLIVEIRA; RAMOS, REBECCA CAPERA; POSSIDONIO, GEOVANA; BOSCULO, MARIA RACHEL MELO; OLIVEIRA, PAULA LIMA; COSTA, LETICIA RAMOS; ZAMBONI, VINICIUS AQUILES GOMES; MARQUES, MARCEL GAMBIN; DE ALMEIDA, BRENO FERNANDO MARTINS. Feline obesity causes hematological and biochemical changes and oxidative stress - a pilot study. VETERINARY RESEARCH COMMUNICATIONS, v. 47, n. 1, p. 11-pg., 2022-07-02. (18/18220-0, 18/19683-4)