Influence of 10% teriparatide-functionalized bioglass, produced sonochemically, in the osteogenesis and in the bone tissue biomechanical

• Grant number: 19/07636-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2019
• Effective date (End): April 30, 2020
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luana Marotta Reis de Vasconcellos
• Grantee: Leonardo Alvares Sobral Silva
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

With the increase in the life expectancy of the population, there was also an increase in the index of bone-metabolic diseases, with that it increased the search for strategies for regeneration and tissue repair bone. The objective of this study will be to evaluate the influence the functionalization of bioglass with drug teriparatide in the differentiation and activity of mesenchymal cells and in the bone repair in critical defects performed in ovariectomized rats, aiming at orthopedic and odontological application. For the in vitro study, osteoblasts obtained from mesenchymal cells isolated from femurs ovariectomized rats were used. After differentiation, cells will be submitted to cell viability tests, total protein content, alkaline phosphatase activity (ALP), formation of mineralization nodules. The cellular interaction with the biomaterial will be evaluated by means of cell morphology after 5 days. In the in vivo study, 18 Wistar rats will be used, which will initially be divided into two groups (n = 09), a bilateral ovariectomy (OVX) group and a simulated ovariectomy surgery group (Sham). After 30 days of these procedures, regardless of the group, all rats will be submitted to 3.0 mm critical bone defects on tibia. The defects total number will be 18 for OVX and Sham, and these will be randomized distributed among the filled methods: clot (C), bioglass (BG) and bioglass associated with 10% teriparatide (BGT), will be 6 bone defects by filled in each groups (OVX e Sham). After 4 weeks, the animals will be euthanized, with n = 6 for each subgroup (BV and BVT), and the area of bone repair at calvaria will be assessed by histological, histomorphometric and immunohistochemistry. The bone defects sites will be submitted to flexural resistance test by means of three-point test. The data will be statistically analyzed, initially by means of the normality test aiming the application of the appropriate statistical test. The level of significance adopted will be the conventional value of 5%.