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Generation and characterization of gemcitabine-resistant pancreatic carcinoma-derived cell lines

Grant number: 19/04420-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2019
Effective date (End): December 31, 2019
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Eduardo Moraes Rego Reis
Grantee:Victoria de Paiva Oliveira
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The project aims to generate immortalized PDAC cell lines resistant to gemcitabine, and the molecular characterization of this resistance. Such lines will be generated by prolonged treatments under sub-lethal concentrations of the drug and will have their resistance evaluated by determining the expression of resistance marker genes already described in the literature by RT-qPCR. In addition to widely studied PDAC lines, a line derived from PDAC xenotransplantation will also be used, which more reliably recapitulates the patient's tumor microenvironment. The results obtained in this project will be the basis for a project under development in the Laboratory of Integrated Research in Cancer of the IQ-USP, which aims to perform the scan of components of the epigenetic machinery functionally associated with chemoresistance and sensitivity to gemcitabine in cancer of pancreas.