Experimental study of the renin-angiotensin-aldosterone system participation in hemodynamic and morphhofunctional cardiac adaptations caused by the ovarian failure: effect of aerobic physical training

• Grant number: 19/03431-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2019
• End date: February 28, 2021
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Hugo Celso Dutra de Souza
• Grantee: Thauane Hanna Fares
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Deprivation of ovarian hormones in young rats is marked by an important increase in cardiac fibrosis, accompanied by adverse cardiac remodeling. However, the mechanisms are still uncertain. Thus, we will investigate the involvement of the renin-angiotensin-aldosterone system (RAAS) in these findings, and whether the mechanisms involved are linked to increased activity and expression of matrix metalloproteinase-2 (MMP-2) and calpain-1. In addition, we will also investigate the role of aerobic physical training as moderator of this process. Sixty-four Wistar rats were submitted to ovariectomy at the 10th week of life and were divided into two groups: a group of 22-week-old sedentary rats (N= 32) and a group of trained rats (N= 2) during the last 10 weeks (12th to 22nd week of life). Each large group will be subdivided into two smaller groups; treated with vehicle (N =16) and treated with enalapril maleate (10mg/kg/day, N=16), an angiotensin II converting enzyme inhibitor. All animals will be submitted to the following experimental protocols; hemodynamic evaluation will consist of recording the evolution of blood pressure (BP) and heart rate (HR) by means of plethysmographic examination at four different times, 12th, 14th, 18th and 22nd weeks of life; echocardiographic examination at the 12th and 22nd weeks of life to assess cardiac morphology and functionality; evaluation of the activities of calpain-1 and MMP-2 using gel zymography, and protein expression by means of western blot and immunofluorescence techniques. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FARES, Thauane Hanna. Experimental study of the participation of the renin-angiotensin-aldosterone system in cardiac hemodynamic and morphofunctional adaptations caused by ovarian failure - effect of aerobic exercise training. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.