Effect of omega-3 polyunsaturated fatty acids supplementation on the cognitive fuction and metabolomic of young dogs

• Grant number: 19/01347-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2019
• End date: August 31, 2020
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Márcio Antonio Brunetto
• Grantee: Roberta Bueno Ayres Rodrigues
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The polyunsaturated fatty acids (PUFAs) of the most important omega-3 family are linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). DHA occurs in high concentrations in the brain and plays an important role in the development and function of the nervous system. Diets with sources rich in DHA, such as fish oil, contribute to the increased deposition of this fatty acid in the neural membranes. It is also known that dietary interventions can alter cell function and the production of metabolites. The objective of the present proposal is to evaluate the effect of DHA concentrate fish oil supplementation on the cognitive and metabolomic function of growing dogs. Dogs were diveded randomly into two groups of six Animals: Control Group (GC), without fish oil supplementation, and Test Group (GT) with daily supplementation of 30 mg DHA/kg body weight. Food consumption, body, muscle and faecal score, cognitive function, metabolic characterization, serum fatty acid concentration and total antioxidant capacity will be evaluated before (T0) and after 30 (T30), 60 (T60) and 90 (T90) days of supplementation. Residual normality and homogeneity of variances will be verified by the Shapiro Wilk and Levene test, respectively. The Chi-square test will be used to evaluate the cognitive function. The Principal Component Analysis (PCA), and the use of Chenomix RNM suite 7.7 software will aid in the identification of metabolites. The quantitative values will be evaluated by ANOVA with measures repeated in time, when they obey the statistical premises and in case of non-parametric data, the Wilcoxon test will be used. The software used will be the Statistical Analysis System (SAS, version 9.4). Values of p <0.05 will be considered significant. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RODRIGUES, Roberta Bueno Ayres. Effects of omega-3 polyunsaturated fatty acid supplementation on the cognitive function and metabolomic of growing dogs. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.