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Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels

Grant number: 18/26470-7
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Start date: June 01, 2019
End date: July 19, 2024
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Maria Bernadete Amâncio Varesche
Grantee:Camila Abreu Borges da Silva Rabelo
Host Institution: Escola de Engenharia de São Carlos (EESC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:22/10615-1 - Consolidation of the biorefinery concept applied to the biological treatment of wastewater and solid wastes, AP.TEM
Associated scholarship(s):20/04000-9 - Fractionation of residues from vineyard and wine-making subproducts, BE.EP.PD

Abstract

This project aims to apply two differential techniques in the bioconversion of sugarcane bagasse (SCB) in hydrogen, methane and organic acids. The first technique will be the application of the laccase enzyme to the SCB saccharification and the second will be the quantification of the number of copies of hydrogenase gene by quantitative real time Polymerase Chain Reaction (qPCR) and gene expression by reverse transcription and quantitative Real Time Polymerase Chain Reaction (RT-qPCR) during the SCB bioconversion by pure culture. Therefore, we will seek new partnerships with the Laboratory of Functional and Structural Biochemistry of UFSCar and with the Laboratory of Animal Health of Embrapa to make them feasible. From this, the proposed methodology is composed of 5 stages. Step 1 will consist of the laccase extraction from the yeast Pichia pastoris. In step 2, the laccase will be applied as SCB enzymatic pretreatment, and the substrate concentration, pH and temperature factors will be optimized by factorial design. In step 3, the fermentation of hydrolyzed SCB by pure culture of Paraclostridium benzoelyticum will be performed and during this process, the expression of hydrogenase enzymes will be monitored and compared with the metabolites production. In step 4, a study will be carried out to replace the liquid medium of the batch to increase the production of hydrogen. And finally, in step 5, the batch effluents from step 4 will be used to produce methane with the addition of methanogenic inoculum. It is expected with the results obtained in the present study, to present a new viable possibility for hydrolysis of SCB. It is intended to use and make feasible the use of qPCR and RT-qPCR for the monitoring of fermentation processes of biofuels production. These possibilities are still not routinely carried out in the Biological Processes Laboratory, so the partnership with Embrapa and UFSCar will be extremely valuable to aggregate knowledge in new research fronts. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DIONIZIO, BRUNA SOARES; SILVA RABELO, CAMILA ABREU B.; RAMOS DE JESUS, HUGO CESAR; AMANCIO VARESCHE, MARIA BERNADETE; FERREIRA DE SOUZA, DULCE HELENA. The Deconstruction of the Lignocellulolytic Structure of Sugarcane Bagasse by Laccases Improves the Production of H-2 and Organic Acids. Applied Biochemistry and Biotechnology, v. 194, n. 7, p. 22-pg., . (18/26470-7, 17/15455-4, 18/06297-9)
BOTTA, LIVIA SILVA; DELFORNO, TIAGO PALLADINO; SILVA RABELO, CAMILA ABREU B.; SILVA, EDSON LUIZ; AMANCIO VARESCHE, MARIA BERNADETE. Microbial community analyses by high-throughput sequencing of rumen microorganisms fermenting office paper in mesophilic and thermophilic lysimeters. PROCESS SAFETY AND ENVIRONMENTAL PROTECTION, v. 136, p. 182-193, . (13/04200-4, 18/26470-7)
MOURA, A. G. L.; RABELO, C. A. B. S.; OKINO, C. H.; MAINTINGUER, I, S.; SILVA, E. L.; VARESCHE, M. B. A.. Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: Effects on hydA expression. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 45, n. 53, p. 28447-28461, . (18/26470-7, 17/23008-8, 15/06246-7)
SILVA RABELO, CAMILA ABREU B.; OKINO, CINTIA HIROMI; SAKAMOTO, ISABEL KIMIKO; AMANCIO VARESCHE, MARIA BERNADETE. Isolation of Paraclostridium CR4 from sugarcane bagasse and its evaluation in the bioconversion of lignocellulosic feedstock into hydrogen by monitoring cellulase gene expression. Science of The Total Environment, v. 715, . (18/26470-7, 15/06246-7)
MOURA, ALANA G. L.; DELFORNO, TIAGO P.; RABELO, CAMILA A. B. S.; SILVA, EDSON L.; VARESCHE, MARIA B. A.. Iron zero valent nanoparticles applied in the biorefinery concept: Functional genes in continuous bioreactor fermenting vinasse. BIOMASS & BIOENERGY, v. 174, p. 14-pg., . (17/23008-8, 15/06246-7, 18/26470-7)
SILVA RABELO, CAMILA A. B.; DIONIZIO, BRUNA S.; MOURA, ALANA G. L.; MALAGO-, WILSON, JR.; VARESCHE, MARIA BERNADETE A.. Dilution strategy to obtain H-2 in the 2nd acidogenic phase from enzymatically pre-treated sugarcane bagasse. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v. 47, n. 76, p. 18-pg., . (15/06246-7, 18/26470-7)
MOURA, A. G. L.; DELFORNO, T. P.; RABELO, C. A. B. S.; KUMAR, G.; SILVA, E. L.; VARESCHE, M. B. A.. Iron and nickel nanoparticles role in volatile fatty acids production enhancement: Functional genes and bacterial taxonomy in an anaerobic fluidized bed reactor. Biochemical Engineering Journal, v. 187, p. 12-pg., . (17/23008-8, 15/06246-7, 18/26470-7)
MOURA, ALANA G. L.; RABELO, CAMILA A. B. S.; SILVA, EDSON L.; VARESCHE, MARIA BERNADETE A.. Application of nanoparticles to increase biological hydrogen production: the difference in metabolic pathways in batch and continuous reactors. ENVIRONMENTAL TECHNOLOGY, v. N/A, p. 9-pg., . (17/23008-8, 15/06246-7, 18/26470-7)