Evaluation of the potential of osteogenic differentiation and oxidative stress of mesenchymal stem cells derived from bone marrow in contact with porous titanium alloy

• Grant number: 19/09752-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2019
• End date: August 31, 2020
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Liz Garcia Alves
• Grantee: Carolina da Silva Dias
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

One of the most surgical challenges in orthopedics is that the stem cells that are attracted to the injured region are able to differentiate into bone tissue and promote the integration of the prosthesis with the new-formed bone. Well-known that titanium (Ti) alloys are indicated because of their superior strength compared to another alloy. In this way, aiming to the ability of stem cells derived from equine bone marrow (bmMSCs) to differentiate under the direct and indirect contact of a new Ti alloy by the possible release of metallic ions will be evaluated. The evaluation of the differentiation of the bmMSCs will be analyzed by the osteogenic differentiation by indirect contact with the Ti alloy, in order to estimate if the biomaterial can release particles in the medium, that affect or not the differentiation process. Already the differentiation osteogenic by direct contact with the Ti alloy, will be performed with the plating of the bmMSCs on the material surface and analyzed by Scanning Electron Microscopy. Both differentiations (indirect and direct) will be evaluated at the moments: 24h,3, 5, 10 and 15 days. Confirmation of the differentiation will be made by Alizarin Red staining and by the colorimetric Alkaline Phosphatase assay. In addition, there will be an in situ evaluation of oxidative stress in 0h, 3h 12h, 24h, 48h and 7 days of bmMSCs with biomaterial conditioned, through the measurement of antioxidant enzymes (HRP and SOD) by an electroanalytical biosensor. This analysis is extremely important because an environment in hypoxia is closely linked with biological functions, such as proliferation, differentiation, adhesion, cell death, and consequent tissue repair. Finally, the quantitative data obtained will undergo a normality test and analysis of variance. Significant differences when P is <0.05.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, CAROLINA DA SILVA; ROSSI, MARIANA CORREA; APOLONIO, EMANUEL V. P.; ROSA, GUSTAVO DOS SANTOS; HUBBE PFEIFER, JOAO PEDRO; HUSSNI, CARLOS ALBERTO; WATANABE, MARCOS JUN; ALVES, ANA LIZ GARCIA. Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 32, n. 12, DEC 2021. (19/09752-1, 17/14460-4, 17/13876-2)