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Inflammatory markers of saliva and gingival sulcus of teeth to receive metal-ceramic crown measured in different stages of clinical procedures and the influence of renin-angiotensin system on treatment

Grant number: 19/10344-5
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): July 01, 2019
Effective date (End): January 31, 2022
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Carlos Ferreira dos Santos
Grantee:Isadora Prado Cano
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated research grant:15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM


The aim of any restorative procedure is to reestablish/maintain function, aesthetics, phonetics and periodontal tissues health. In fixed prosthodontics, the main reason of biological failure is caries as 32% of the cases, while periodontal disease cause 4% of such failures, the frequency for endodontic problems. The objective of the present study is to determine whether the blockage of renin-angiotensin components and the dental materials conventionally used for rehabilitation with metal-ceramic crowns influence qualitatively and quantitatively the release of inflammatory-related cytokines, the bone metabolism in gingival sulcus, salivary secretion and its inflammatory markers. The sample will consist in 51 patients in need of prosthodontic restauration in premolars, with total metal-ceramic crowns, divided into 3 groups (n=17). The control group will be characterized as normotensive and systemically healthy patients. The other two test groups will consist of hypertensive-treated patients and divided by their treatments: whether with AT1 receptor for angiotensin II blockers (ATB) or with Angiotensin Converting Enzyme inhibitors (ACEi). The analyzed parameters will be: the presence of pro and anti-inflammatory cytokines in gingival sulcus of different times of clinical phases: 1) 30 days after dental and root scaling; 2) 2 and; 3) 15 days after temporary crowns cementation; 4) 2 days; 5) 15 days and; 6) 6 months after metal-ceramic crowns cementation. The clinical evaluations will be: probing depth, clinical attachment level, plaque index, gingival recession, gingival index, width and thickness of keratinized mucosa. Salivary flux will be measured in the last day of control pos-cementation; total protein content and inflammatory markers in saliva will be measured 15 days after temporary and final cementation. The quantification of inflammatory markers will be registered as averages and standard deviations, and data normality will be assured through Kolmogorov-Smirnov method. Analyses will also be performed in contralateral premolars that will serve as the own patient control for non-treated teeth. For time-related comparison, quantitative analyses will be performed with paired "T" test and for score variables will be performed non-parametric test of Wilcoxon. Differences will be considered statistically significant when results are p<0.05. (AU)