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Study of different internal implant/abutment connections: stress transfer and bacterial sealing capability under static and post thermomechanical loading conditions

Abstract

The use of implants for rehabilitation of totally or partially edentulous patients has been increasing and with great success rate. However, several studies indicate that there is bi-directional bacterial microleakage through the implant / abutment interface and this may compromise the long-term success of implants. The aim of the present study will be to evaluate the passage of bacterial species from human saliva into the implants through the implant / prosthetic abutment interface after accelerated mechanical fatigue simulation using the DNA sequencing technique. For this study will be used internal triple channel tapered internal connection implants associated with universal, solid and through-bolt abutments, UCLAs and titanium bases, from three different manufacturers. Each part of the study (3) will relate to a factory system with no mix between manufacturers. The implant / abutment assemblies will be torqued according to each manufacturer's recommendations. For titanium and CoCr bases, custom abutments will be produced. Then each set will be scanned and resin crowns will be obtained by 3D printing. The crowns will be cemented with temporary cement. Then the sets will be scanned in microCT for interface evaluation. Subsequently, they will be immersed in human saliva and subjected to mechanical fatigue testing, 2 X 106 cycles, at 5Hz frequency and 200N load, with crown sliding. The load will be applied to the crown incisal with an inclination of 30º to the long axis of the implant and during the test, the specimens will be simultaneously thermally cycled between 5º and 55º C. After cycling, the sets will be removed from the test machine. , externally disinfected and resubmitted to microCT. Immediately afterwards, the contents of the interior of the implants and the material present in the threads of the fixation screws of their respective prosthetic connectors will be collected and the samples will be processed by the 16S bacterial ribosomal gene pyrosequencing technique. The data obtained will be submitted to the appropriate statistical analysis. (AU)

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