Study of different internal implant/abutment connections: stress transfer and bacterial sealing capability under static and post thermomechanical loading conditions

• Grant number: 19/25405-0
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2021
• End date: May 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Ricardo Faria Ribeiro
• Grantee: Ricardo Faria Ribeiro
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• City of the host institution: Ribeirão Preto
• Associated researchers: Adriana Cláudia Lapria Faria ; Cássio Do Nascimento ; IZABELA CRISTINA MAURICIO MORIS RIVERA ; Renata Cristina Silveira Rodrigues Ferracioli

Abstract

The use of implants for rehabilitation of totally or partially edentulous patients has been increasing and with great success rate. However, several studies indicate that there is bi-directional bacterial microleakage through the implant/abutment interface and this may compromise the long-term success of implants. The aim of the present study will be to evaluate the passage of bacterial species from human saliva into the implants through the implant / prosthetic abutment interface after accelerated mechanical fatigue simulation using the DNA sequencing technique. For this study will be used internal triple channel tapered internal connection implants associated with universal, solid and through-bolt abutments, UCLAs and titanium bases, from three different manufacturers. Each part of the study (3) will relate to a factory system with no mix between manufacturers. The implant/abutment assemblies will be torqued according to each manufacturer's recommendations. For titanium and CoCr bases, custom abutments will be produced. Then each set will be scanned and resin crowns will be obtained by 3D printing. The crowns will be cemented with temporary cement. Then the sets will be scanned in microCT for interface evaluation. Subsequently, they will be immersed in human saliva and subjected to mechanical fatigue testing, 2 X 106 cycles, at 5Hz frequency and 200N load, with crown sliding. The load will be applied to the crown incisal with an inclination of 30º to the long axis of the implant and during the test, the specimens will be simultaneously thermally cycled between 5º and 55ºC. After cycling, the sets will be removed from the test machine. , externally disinfected and resubmitted to microCT. Immediately afterwards, the contents of the interior of the implants and the material present in the threads of the fixation screws of their respective prosthetic connectors will be collected and the samples will be processed by the 16S bacterial ribosomal gene pyrosequencing technique. The data obtained will be submitted to the appropriate statistical analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMIONATO, ANSELMO AGOSTINHO; SANTOS, EMERSON DE SOUZA; FARIA, ADRIANA CLAUDIA LAPRIA; DO NASCIMENTO, CASSIO; RODRIGUES, RENATA CRISTINA SILVEIRA; RIBEIRO, RICARDO FARIA. Bacterial Detection, Deformation, and Torque Loss on Dental Implants with Different Tapered Connections Compared with External Hexagon Connection after Thermomechanical Cycling. MATERIALS, v. 16, n. 11, p. 20-pg., 2023-05-23. (19/25405-0)