Titanium-enriched medium in shear-stressed endothelial cells: look on cell adhesion, survival and angiogenesis

• Grant number: 19/09140-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2019
• End date: January 19, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Willian Fernando Zambuzzi
• Grantee: Bruna Rodrigues Martins
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration, AP.JP

Abstract

Biomaterials have been the focus of research in the field of health, mainly because of their increasing importance for improving quality and human longevity. Although already is well established, the molecular and cellular effect of titanium is not yet fully understood. Our research group has worked extensively in the attempt to understand osteoblast adhesion on biomaterials. However, peri-implant healing and repair events require a recapitulation of cellular and molecular events, and endothelial cells are important in this scenario, and the shear stress model must be considered. As angiogenesis does not occur, in principle, juxtaposed to an implant surface, it is a rational process is an indirect effect within the surrounding tissue. To address this issue, we shall incubate titanium-based implants into cell culture mediums up to 24 hours, and later this medium will be used to treat endothelial cells up to 24 hours when the biological samples will be harvested to 1. Quantify titanium and aluminum without conditioned conditioning through a graphite furnace; 2. Investigation of the biological behavior of endothelial cells, by checking Integrin, FAK, Src, and Cofilin; 3. selection of response responses to treatment with titanium - Akt, MAPKs, VEGF and VEGFR; 4. Effect of conditioned medium on in vitro angiogenesis events.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS, BRUNA RODRIGUES; PINTO, THAIS SILVA; DA COSTA FERNANDES, CELIO JUNIOR; BEZERRA, FABIO; ZAMBUZZI, WILLIAN FERNANDO. PI3K/AKT signaling drives titanium-induced angiogenic stimulus. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 32, n. 1, JAN 27 2021. (14/22689-3, 19/09140-6)

PINTO, T. S.; MARTINS, B. R.; FERREIRA, M. R.; BEZERRA, F.; ZAMBUZZI, W. F.. Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis. BIOMED RESEARCH INTERNATIONAL, v. 2022, p. 11-pg., 2022-02-23. (19/26854-2, 19/09140-6)